通过特异PCR扩增和16S rDNA序列分析检测动弯杆菌  

Detection of Mobiluncus spieces by special primer amplification and 16S DNA sequence analysis

作　　者：何亮[1] 陈群[2] 曾忠铭 刘志恒[1] 黄英[1] 

机构地区：[1]中国科学院微生物研究所,北京100080 [2]广东医学院微生物学教研室,湛江524023 [3]深圳南山医院微生态实验室,深圳518000

出　　处：《微生物学报》2005年第1期27-30,共4页Acta Microbiologica Sinica

摘　　要：细菌性阴道病 (BacterialVaginosis,BV)是由于细菌过度生长所致阴道微生态非正常改变 ,从而导致的一类多微生物病 (PolymicrobialDiseases)。动弯杆菌 (Mobiluncussp .)与BV发生有密切关系 ,但该菌为厌氧菌 ,营养要求苛刻 ,很难进行纯培养 ,国内鲜有研究报道。本文先对BV动物模型恒河猴阴道分泌物进行厌氧菌混合培养 ,抽提混合物染色体DNA ,之后设计了一对动弯杆菌 16SrRNA基因的特异性引物 ,用PCR的方法扩增出了特异片段。通过对扩增产物进行测序分析 ,确定检测出的为动弯杆菌 。Bacterial Vaginosis (BV), a microecological disease leaded by overgrowth of the vaginal bacteria, is one of the Polymicrobial Diseases. The close relationship between BV and Mobiluncus ssp. was recognized gradually. But It is difficult to get the pure culture of this anaerobic bacterium because of its rigorous requirement for growth conditions. The vaginal discharge came from the BV animal model-Rhesus monkey was cultured in anaerobic environment. The 16S rRNA gene was amplified and sequenced using mobiluncus-specific primers. Mobiluncus ssp, closely related to Mobiluncus mulieris, were detected,by comparing with the 16S rRNA genes in the GenBank.

关 键 词：混合培养 特异性PCR 16S rRNA序列分析 动弯杆菌 细菌性阴道病 

分 类 号：R446.5[医药卫生—诊断学]