Mn^(2+)对必特螺旋霉素产生菌代谢和必特螺旋霉素合成的影响  

Influence of Mn^(2+) on the biotechmycin fermentation

作　　者：康源[1] 王永红[1] 庄英萍[1] 储炬[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心(上海),上海200237

出　　处：《微生物学报》2005年第1期81-85,共5页Acta Microbiologica Sinica

基　　金：国家"8 63计划"( 2 0 0 2AA2 170 2 1);国家重大科技专项 ( 2 0 0 2AA2Z3 45 1)~~

摘　　要：通过在必特螺旋霉素产生菌WSJ 1 195发酵过程中添加金属离子Mn2 + 发现 :发酵前期 (2 4h左右 )添加Mn2 + 可以明显提高生物效价 ,加入的Mn2 + 浓度以 5mmol L为最佳。实验显示添加Mn2 + 后发酵液pH逐渐下降 ,整个产素期间pH一直低于对照 ;与对照相比添加Mn2 + 摇瓶菌体浓度也较低。通过研究必特螺旋霉素发酵过程有机酸的变化趋势发现 :2 4h添加 5mmol LMn2 + 后发酵过程中有机酸含量已经发生变化 ,其中丙酸浓度的增长最为显著 ,84h时其浓度为对照的 6倍。通过丙酸盐的添加实验证实了发酵前期添加Mn2 + 可以促进产物合成的原因之一是促进了丙酸等前体酸的合成 。The effect of Mn 2+ on the biotechmycin fermentation by Bioengineered strain WSJ-l-195 was studied. In the fermentation process, Mn 2+ could improve the biological potency significantly, especially when Mn 2+ concentration was 5mmol/L added at 24h.The pH profile of fermentation broth decreased gradually after 5mmol/L Mn 2+ supplemented at 24h, and PMV was lower than that of the control sample. Further research about the influence of Mn 2+ on the biosynthesis of biotechmycin was carried out in the aspect of organic acids. The results showed that concentrations of organic acids in a fermentation with 5mmol/L Mn 2+ supplemented at 24h had been changed greatly, especially the concentration of propionic acid, of which the highest value was about 6 times as that in the control sample at 84h. In addition, it was found that the yield of biotechmycin could be improved significantly with tiny amount of propionic acid added. Therefore, it can be concluded that Mn 2+ has profound influence on the biosynthesis of biotechmycin: it enriches the biotechmycin precursor pool such as propionic acid and thus improves the yield of biotechmycin.

关 键 词：锰离子 必特螺旋霉素 有机酸 代谢 WSJ-1-195菌株 发酵 生物合成 大环内酯类抗生素 

分 类 号：TQ465[化学工程—制药化工]