角质形成细胞μ-阿片受体表达的研究  

The expression of mu-opiate receptor in human keratinocyte cell lines

作　　者：毕新岭[1] 顾军[1] 米庆胜[2] 田中伟[3] 

机构地区：[1]第二军医大学长海医院皮肤科,上海200433 [2]泰山医学院附属医院皮肤科,山东泰安271000 [3]西安交通大学第二医院皮肤科,陕西西安710004

出　　处：《临床皮肤科杂志》2005年第3期137-138,共2页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助项目(30271197)

摘　　要：目的:研究不同特性的角质形成细胞μ-阿片受体的表达情况。方法:以体外培养的角质形成细胞株HaCaT细胞、人鳞状细胞癌(简称鳞癌)细胞株A431、神经母细胞瘤SK-N-SH细胞株为对象,采用逆转录(RT)-PCR方法研究细胞μ-阿片受体的表达。结果:在常规体外培养条件下,角质形成细胞株HaCaT细胞、人鳞癌细胞株A431的RT-PCR结果显示有μ-阿片受体mRNA的表达,后者的表达水平略高于前者。结论:μ-阿片受体在角质形成细胞的表达,为神经系统和皮肤通过神经肽直接发生作用提供依据。Objective: To study the expression of mu-opiate receptor in different human cell lines. Methods: The cells of human epidermal keratinocyte cell line (HaCaT), human squamous carcinoma cell line (A431) and neuroblastoma cell line (SK-N-SH) were cultured in a conventional culture-condition. The expression of mu-opiate receptor in cell lines was assessed by RT-PCR method. Results: The expression of mu-opiate receptor mRNA was found in the HaCaT and A431, and the expression level was higher in A431 than that in HaCaT. Conclusion: The expression of mu-opiate receptor mRNA in the keratinocytes indicates that the nervous system may interact with the skin by the neuropeptides.[Key words] keratinocytes; mu-opiate receptor

关 键 词：角质形成细胞 Μ-阿片受体 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R392.11[医药卫生—基础医学]