成年大鼠脑内ATP敏感性钾通道亚型mRNA表达的研究  

EXPRESSION OF ATP-SENSITIVE POTASSIUM CHANNEL SUBUNITS IN ADULT RAT BRAIN

作　　者：王刚[1] 陈生弟[1] 杜芸兰[1] 范国华[1] 陆国强[1] 

机构地区：[1]上海第二医科大学附属瑞金医院神经科,神经病学研究所上海200025

出　　处：《神经解剖学杂志》2005年第1期29-33,共5页Chinese Journal of Neuroanatomy

基　　金：国家重点基础研究规划"脑功能和脑重大疾病的基础研究"(G1999054000 );国家自然科学基金 (No. 30171025 );上海市卫生系统"百人计划"(97BR001)资助项目

摘　　要：为探讨ATP敏感性钾通道(KATP)亚型在大鼠脑组织中的表达,本研究采用半定量逆转录聚合酶链反应 (RT PCR)检测了大鼠小脑、大脑皮质、海马、纹状体、黑质的KATP亚型mRNA的表达。结果显示Kir6. 1、Kir6. 2、Sur1、Sur2B在小脑、大脑皮质、海马、纹状体、黑质中均有表达,Sur2A在脑组织中未见表达。Kir6. 1mRNA在海马和黑质的相对表达水平明显高于小脑、大脑皮质和纹状体(P<0.01);Kir6. 2和Sur1mRNA在黑质的相对表达水平明显高于小脑、大脑皮质、海马和纹状体 (P<0.01 );Sur2BmRNA在黑质、海马和纹状体的相对表达水平明显高于小脑和大脑皮质(P<0.01 )。以上结果提示KATP在脑内具有广泛表达,其表达水平在不同部位存在着差异性。In order to elucidate the expression of ATP-sensitive potassium channel (K_ ATP) subunits in normal rat brain, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect the expressions of Kir6.1, Kir6.2, Sur1, Sur2A and Sur2B in cerebellum, cerebral cortex, hippocampus, striatum and substantia nigra, respectively. The results revealed that except Sur2A, the other four subunits of K_ ATP were expressed in all above-mentioned regions. The relative mRNA expression levels of Kir6.1 in the substantia nigra and hippocampus were higher than those in cerebellum, cerebral cortex and striatum (P<0.01). The expression of Kir6.2 and Sur1 mRNA in the substantia nigra was higher than that in cerebellum, cerebral cortex, hippocampus and striatum (P<0.01). Our study indicates that K_ ATP subunits are widely expressed in rat brain with different expression levels in different brain regions.

关 键 词：黑质 纹状体 大脑皮质 小脑 海马 表达水平 亚型 RNA RT—PCR ATP敏感性钾通道 

分 类 号：R346[医药卫生—基础医学]