Quantitation of Genital Herpes Virus DNA by Polymerase Chain Reaction and ELISA  

作　　者：程培华 

机构地区：[1]Department of Dermatology, Affiliated Hospital Guilin Medical College

出　　处：《Chinese Journal of Sexually Transmitted Infections》2002年第1期27-30,共4页中华性传播感染杂志（英文版）

摘　　要：Objective: To detect and quantitate genital herpes simplexvirus (HSV) DNA in specimens from 100 patients clinicallydiagnosed with genital herpes. Methods: Polymerase Chain Reaction (PCR) andenzyme-linked immunosorbent assay (ELISA) were used witha standard curve of DNA copies of HSV as quantitativecontrast. Results: Ninety-three cases were confirmed HSV positiveand 7 cases were found to be negative. There were 58 cases ofHSV-2 (62.4%) and 35 cases of HSV-1(37.6%) among the 93positive cases. The number of DNA plasmids ranged from 115to 1.1×10~5 per 250μL among the 93 positive samples (mean=7.1×10~4/250μL). The number of HSV DNA plasmids rangedfrom 136 to 1.1×10~5 copies per 250μL(mean=7.6×10~4) amongthose with HSV-2, and 115 to 9.4×10~4 per 250μL(mean=6.3×10~4) among those with HSV-1. Meanwhile 10μL ofextracted and dissolved DNA randomly taken from 8 each ofHSV-2 and HSV-1 samples were tested. The number of HSV-2DNA plasmids ranged from 35 copies to 2.7×10~4 (Mean=1.8×10~4) and the number of HSV-1 DNA ranged from 29 to2.5×10~4 (Mean=1.6×10~4). In the 7 negative cases, the quantityof HSV plasmids was zero. Conclusion: The sensitivity of ELISA quantitation (93%) isequal to that of Southern blot. The sensitivity of PCR fordiagnosis is 91%, and 88% for PCR typing.Objective:To detect and quantitate genital herpes simplex virus (HSV) DNA in specimens from 100 patients clinically diagnosed with genital herpes. Methods: Polymerase Chain Reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were used with a standard curve of DNA copies of HSV as quantitative contrast. Results: Ninety-three cases were confirmed HSV positive and 7 cases were found to be negative. There were 58 cases of HSV-2 (62.4%) and 35 cases of HSV-1 (37.6%) among the 93 positive cases. The number of DNA plasmids ranged from 115 to 1.1×l0^5 per 250pL among the 93 positive samples (mean =7.1×10^4/250μL). The number of HSV DNA plasmids ranged from 136 to 1.1×l0^5 copies per 250pL (mean =7.6×10^4) among those with HSV-2, and 115 to 9.4×10^4 per 250pL (mean =6.3×10^4) among those with HSV-1. Meanwhile 10μL of extracted and dissolved DNA randomly taken from 8 each of HSV-2 and HSV-1 samples were tested. The number of HSV-2 DNA plasmids ranged from 35 copies to 2.7×10^4 (Mean=l.8×10^4) and the number of HSV-1 DNA ranged from 29 to 2.5×10^4 (Mean = 1.6×10^4). In the 7 negative cases, the quantity of HSV plasmids was zero. Conclusion: The sensitivity of ELISA quantitation (93%) is equal to that of Southern blot. The sensitivity of PCR for diagnosis is 91%, and 88% for PCR typing.

关 键 词：HSV DNA quantitation PCR ELISA Southern blot 

分 类 号：R373[医药卫生—病原生物学] R446.61[医药卫生—基础医学]