6-巯基嘌呤滤纸基质室温磷光光谱研究  

Study on Paper Substrate Room Temperature Phosphorescence of 6-Mercaptopurine

作　　者：丁莉华[1] 董川[1] 双少敏[1] 

机构地区：[1]山西大学化学化工学院,太原市坞城路36号030006

出　　处：《光谱实验室》2003年第6期830-833,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金 (No.2 0 172 0 35 );山西省自然科学基金 (No.2 0 0 110 10 )资助项目

摘　　要：本文建立了 6 -巯基嘌呤 (6 - MP)的滤纸基质室温磷光 (PS- RTP)检测法 ,实验条件已经最佳化。研究表明 ,6 - MP的 PS- RTP光谱的最大激发波长 λex与最大发射波长 λem分别为 312 nm和 4 5 5 nm。30多种无机盐类重原子微扰剂对 6 - MP的 RTP影响的研究结果表明 ,Cd盐可增强 RTP,其中 Cd(OAc) 2 重原子效应最为显著。酸度条件对 6 - MP的 PS- RTP有较大影响 ,在 p H为 7— 8时 RTP发射较强。此外 ,本文也对影响 6 - MP的 PS- RTP的固体基质类型、重原子浓度、干燥条件等进行了详细的研究。实验表明 ,方法的线性范围为 4 .2 6— 10 6 6 ng/spot,检出限为 3.31ng/spot,相关系数为 0 .997,相对标准偏差为 4 .0 7% ,回收率为 99.6 %— 10 0 .5 %。该方法简便、快速、灵敏 ,应用于商业药片的分析 ,结果令人满意。The determination of 6-mercaptopurine (6-MP) have been studied by paper surface room-temperature phosphorescence (PS-RTP). The maximum excitation wavelength is 312nm, and the maximum emission wavelength is 455nm. The effects of more than 30 kinds of heavy atoms on PS-RTP were investigated, and Cd(OAc) 2 is effective heavy atom perturber. 6-MP could emit strong phosphorescence in the range of pH=7-8, and its appropriate acidity is pH=7.7. Various factors affecting the PS-RTP of 6-MP are discussed, including the effects of temperature and drying conditions and so on. Analytical calibration curve of 6-MP are linear in 4.26-1066 ng/spot. The detection limit is 3.31ng/spot, and correlation coefficient is 0.997,and RSD is less than 5%. The recovery is in the range 96.6%-100.5%. The method has been applied to the analysis of commercial tablets with satisfactory results.

关 键 词：6-巯基嘌呤 滤纸基质 室温磷光光谱 6-MP PS-RTP 嘌呤化合物 

分 类 号：O657.39[理学—分析化学]