双抗体夹心法检测冠心病患者血清中肺炎衣原体循环免疫复合物的方法学建立  

Establishment of a novel method of Double Mono-cloned antibody for detection of serum circular immune complex of chlamydia pneumoniae in the patients with coronary heart disease

作　　者：李晶晶[1] 杨占秋[1] 肖红[1] 文莉[1] 

机构地区：[1]武汉大学医学院病毒学研究所,武汉430071

出　　处：《华中医学杂志》2003年第5期241-242,共2页Central China Medical Journal

摘　　要：目的 建立一种敏感而特异的双抗体夹心法检测血液中循环免疫复合物(CIC),并探讨血液中肺炎衣原体(Cpn)CIC与冠心病的关系。方法 包被已知特异性鼠抗人CpnTW183IgG,加入用7％PEG6000,从冠心病患者血清中提取的CpnCIC,再加入酶标二抗与CIC中的抗体结合,通过底物显色检测患者CpnCIC阳性率。结果 冠心病组CpnCIC的阳性率(63.8％)高于对照组(40.0％),差异有显著性意义(X^2=7.78,P<0.05)。结论 建立的双抗体夹心法检测CpnCIC有较高的敏感性和特异性,可用于批量临床标本的检测,血清中中分子量CpnCIC的存在与冠心病之间有密切关系。Objective To establish a sensitive and specific method of double mono-cloned antibody for detection of serum circular immune complex (CIC) and further investigate the relationship between CIC of Chlamydia pneumoniae and the coronary heart disease. Methods The specific mouse anti-human CpnTW183IgG was coated. CpnCIC extracted from the blood of patients with coronary heart disease with 7% PEG6000 was added. Enzyme-labeled second antibody was added to bind with the antibody of CIC. The positive rate of CpnCIC in the patients was measured by the reaction of substrate. Results The positive rate of CpnCIC in the experimental group was higher than in the control group (63.8% vs 40.0%, P<0.05). Conclusion It is a sensitive and specific method of the double layer antibody for detection of CpnCIC. Serum CpnCIC is correlated with coronary heart disease.

关 键 词：双抗体夹心法 冠心病 血清 肺炎衣原体 循环免疫复合物 动脉粥样硬化 AS 

分 类 号：R541.4[医药卫生—心血管疾病] R392[医药卫生—内科学]