N-乙酰-L-半胱氨酸对H_2O_2引起的血管内皮细胞损伤的拮抗效应  

Effects of N-acetyl-L-cystein on endothelial cells injury induce by hydrogen peroxide

作　　者：张静 吴瑜[1] 徐敬根 乔小蓉[3] 王树人[1] 

机构地区：[1]四川大学华西基础医学与法医学院病理生理教研室,四川成都610041 [2]双流县第二人民医院 [3]四川大学分析测试中心

出　　处：《中国呼吸与危重监护杂志》2003年第2期91-94,共4页Chinese Journal of Respiratory and Critical Care Medicine

摘　　要：目的　观察N 乙酰 L 半胱氨酸 (NAC ,痰易净 )对H2 O2 引起的血管内皮细胞损伤的影响。方法　通过测定乳酸脱氢酶 (LDH)释放率和脂质过氧化物 (LPO)值 ,MTT法测定细胞增殖活性 ,流式细胞术测细胞凋亡率 ,研究体外培养的人脐静脉内皮细胞在不同浓度H2 O2 作用下的细胞损伤及凋亡 ,以及NAC对抗细胞损伤及凋亡的效应。结果　随着H2 O2 浓度增加 ,细胞凋亡率明显增高 ,细胞增殖活力降低 ,细胞脂质过氧化物生成增加 ,LDH释放率增加 ,并在一定范围内呈剂量 效应关系。在H2 O2 中同时加入 0 5mmol/LNAC ,可使细胞活力增强 ,凋亡率降低 ,细胞脂质过氧化物生成显著减少 ,LDH释放率降低。结论　NAC可以对抗H2 O2 引起的血管内皮细胞损伤。Objective The study was designed to investigate the protective effects of N acetyl L cystein(NAC) on human endothelial cells injured by hydrogen peroxide (H 2 O 2 ) Methods H 2 O 2 in sequential concentration was added into the cultured human umbilical vein endothelial cells (HUVEC) for 24 hours in serum free medium The proliferative activity of cells (MTT method),the releasing rate of LDH,the lipid peroxidation,and cell apoptosis were assayed.NAC of 0 5 mmol/L was added into the HUVEC co cultured with H 2O 2 for assessment of the antagonistic rate of NAC on H 2 O 2 .Results H 2O 2 increased the cell apoptosis,lipid peroxidation,LDH releasing,decreased the proliferative activity of cell in a H 2O 2 concentration dependent manner These effects of H 2O 2 were inhibited markedly by NAC Conclusion Our data indicated that NAC could prevent human endothelial cells from injury induced by H 2O 2 possibly through its regulation on GSH and GSSG production

关 键 词：N-乙酰-L-半胱氨酸 H2O2 血管内皮细胞损伤 拮抗效应 NAC 痰易净 细胞凋亡 祛痰药 

分 类 号：R965[医药卫生—药理学]