肺炎衣原体感染小鼠肺外脏器病理改变研究  

A histopathological study on the organs other than lungs of the mice with Chlamydia pneumoniae infection

作　　者：印洁[1] 施毅[1] 詹化文[2] 周晓军[2] 朱美英[1] 

机构地区：[1]南京军区南京总医院呼吸科,江苏南京210002 [2]南京军区南京总医院病理科,江苏南京210002

出　　处：《中国呼吸与危重监护杂志》2003年第5期300-302,共3页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：江苏省科委自然科学基金应用基础研究计划赞助(BJ980 6 5)

摘　　要：目的　探讨肺炎衣原体感染小鼠肺外脏器病理改变 ,对肺炎衣原体肺炎的发病机制进行初步的探讨。方法　以肺炎衣原体鼻内或静脉接种Icr小鼠 ,在不同时间点 (1、3、7、14、2 1、2 8及 60d内 )处死动物 ,以HE染色观察小鼠脾脏、肝脏和心脏的病理改变。结果　接种肺炎衣原体后 ,小鼠脾脏中出现中性粒细胞和巨噬细胞浸润 ,小鼠肝脏可见到肝细胞坏死。静脉接种实验组的病理改变比鼻内接种实验组明显。各组小鼠的心脏均未见明显异常改变。结论　肺炎衣原体经静脉接种后 ,肝脏和脾脏均可产生相应的病理改变 ,且较经鼻腔接种者严重。肺炎衣原体主要侵袭肺脏 。Objective To investigate the histopathology of liver,spleen and heart in mice model with Chlamydia pneumoniae ( C pneumoniae ) pneumonia Methods The Icr mice were inoculated with C pneumoniae ,strain CWL 029,by the intranasal or intravenous routes After a single inoculation,mice were killed on the 1st,3rd,7th,14th,21st,28th and 60th day separately Specimens of spleen,liver and heart were obtained from the acute stage of C pneumoniae pneumonitis and stained with hematoxylin eosin Results After inoculation of C pneumoniae ,there were infiltration of neutrophils and macrophages in the spleen and necrosis of hepatocytes The histopathology of iv inoculation group was more serious than that of intranasal inoculation group No pathology changes were observed in heart Conclusions After iv inoculation of C pneumoniae ,the histopathologic changes appeared in the tissue of liver and spleen correspondingly,and the changes were more serious than that of iv inoculation group This study demonstrated that C pneumoniae was mainly limited to lungs and dissemination of C pneumoniae was seldom

关 键 词：肺炎衣原体感染 肺炎衣原体肺炎 发病机制 动物模型 肺外脏器 病理改变 

分 类 号：R56[医药卫生—呼吸系统]