SIV模型猴石蜡包埋淋巴结组织DNA的提取及病毒基因检查  

Isolation of DNA from Paraffin-embedded LymphNode Tissue and the Detection of SIV DNA

作　　者：何伏秋[1] 潘勇[1] 朱华[1] 吴小闲[1] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所

出　　处：《中国实验动物学报》1996年第1期43-47,共5页Acta Laboratorium Animalis Scientia Sinica

摘　　要：从取材于３只ＳＩＶ感染治疗猴和４只ＳＩＶ艾滋病模型猴治疗前后的１１份石蜡包埋淋巴结活检组织中提成基因组ＤＮＡ，分别用ＰＣＲ和巢式ＰＣＲ方法检测ＳＩＶ病毒基因，ＰＣＲ共检出１０份阳性，巢式ＰＣＲ检测１１价样品均阳性，而同期采样的猴外周血标本病毒分离仅３份阳性，ＰＣＲ扩增产物的特异性用限制性内切酶酶切反应得到证实。实验说明，从淋巴结组织中检测ＳＩＶ的病毒基因较外周血病毒分离更能真实地反映病毒感染状况，本研究将对全面和正确评价艾滋病药吻和疫苗治疗效果提供帮助。e detected the SIV DNA in genome extracted from 11 paraffin-embedd-ed lymph node samples of 3 SIV-infected and treated monkeys and 4SAIDS model monkeys before or after treatment by methods of PCR andnested PCR. The resnlts showed that 10 of 11 samples were positive byPCR and all were positive by nested PCR, only 3 of 11 correspondingperiod peripheral blood samples were positive by virus isolation method.In this study, the specificity of PCR prodnction was indentified by BamHIrestrictive digestion. Our experiments proved that the SIV DNA detectionfrom lymph node tissue reflected SIV infection status in monkeys morecorrectly than the virus isolation from the corresponding period peripher-al blood,and provided experimental basis for the evaluation of AIDS drugsand vaccines.

关 键 词：阳性 SIV 病毒基因 石蜡包埋 淋巴结 外周血 艾滋病药物 病毒分离 增产 酶切 

分 类 号：Q78[生物学—分子生物学] R512[医药卫生—内科学]