胶质细胞源性神经营养因子对周围神经再生的作用  

Effect of GDNF on peripheral nerve regeneration in rat

作　　者：陈哲宇[1] 李建红[1] 郑兴东[1] 路长林[1] 何成[1] 

机构地区：[1]上海第二军医大学神经生物学教研室,200433

出　　处：《中国实用手外科杂志》2001年第1期26-28,F003,共4页

基　　金：国家重点基础研究基金资助(国家"973"课题)

摘　　要：目的 研究胶质细胞源性神经营养因子（ｇｌｉａｌ ｃｅｌｌｌｉｎｅ－ｄｅｒｉｖｅｄ ｎｅｕｒｏｔｒｏｐｈｉｃ ｆａｃｔｏｒ，ＧＤＮＦ） 对周围神经再生的作用。方法 硅管套接大鼠坐骨神经，术中一次性将ＧＤＮＦ、生理盐水（ｎｏｒ－ ｍａｌ ｓａｌｉｎｅ ｓｏｌｕｔｉｏｎ，ＳＡＬ）分别加入硅管中。术后４周，应用溃变神经纤维染色法、辣根过氧化物 酶（ｈｏｒｓｅｒａｄｉｓｈ ｐｅｒｏｘｉｄａｓｅ，ＨＲＰ）逆行追踪技术观察 ＧＤＮＦ对轴突再生的影响。结果 与 ＳＡＬ组 相比，ＧＤＮＦ组溃变神经纤维面积明显减少，溃变神经纤维面积百分率由１７．３％降低到１．９％(Ｐ＜０．０１）；ＧＤＮＦ组脊髓标记胞体显著增加，ＨＲＰ标记胞体数的百分率由 ４３．５％上升到 ６８．３％(Ｐ＜０．０１）。结论 外源性ＧＤＮＦ能明显促进周围神经再生。Objective To study the effect of GDNF on peripheral nerve regeneration. Methods Transectioned sciatic nerve in rat was sutured into silicone chamber. GDNF or SAL solution was injected into the silicone chambers during operation. Four weeks later, the effect of GDNF on axonal regeneration was evaluated with use of degenerative neurofiber staining and HRP retrograde tracing. Results Compared with SAL group, the percentage of degenerative neurofiber areas was decreased from 17.3% to 1.9%(P <0.01), and the percentage of labeled spinal intact neuron number was significantly increased from 43.5% to 68.3%(P < 0.01) in GDNF group. Conclusion The results suggested that exogenous GDNF could obviously enhance peripheral nerve regeneration.

关 键 词：胶质细胞源性神经营养因子 周围神经再生 坐骨神经 面神经 腺病毒 

分 类 号：R651.3[医药卫生—外科学]