马尾松磨木木素以及LCC的仿酶降解  

Biomimetic Degradation of MWL and LCC from Masson's Pine

作　　者：欧国隆[1] 谢益民[1] 伍红[1] 顾瑞军[1] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广州510641

出　　处：《中国造纸学报》2000年第B12期68-74,共7页Transactions of China Pulp and Paper

基　　金：国家自然科学基金 !(项目批准号 :2 99740 11);广东省高教厅"千百十工程"优秀人才培训基金 !(992 4)资助项目

摘　　要：采用由Cu2 + /吡啶 /过氧化氢组成的仿酶氧化系统 ,对马尾松 (PinusmassonianaLamb)的磨木木素(MWL)和木素碳水化合物复合体 (LCC)进行仿酶降解 ,通过红外光谱分析以及GC MS分析 ,解析了马尾松MWL和LCC在降解过程中的结构变化 ,并对这种仿酶降解的机理进行了初步探讨。结果表明 ,这种仿酶降解方法对磨木木素和LCC均有较强的降解能力 ,降解后的产物中羰基和羟基增多 ,且木素结构中的部分苯环被开环 ;还有一部分木素降解为香草酸和香草醛等低分子化合物。这说明木素在侧链上受到氧化作用 。Lignin carbohydrate complex (LCC) and milled wood lignin (MWL) prepared from pinus massoniana Lamb were treated with a new delignification system using copper/coordination compound/peroxide. The combination of infrared spectra and GC MS analysis was used to analyze the changes of chemical structure of MWL and LCC during the reaction. The mechanism of this biomimetic delignification was explored. The results showed that the dilignification of MWL and LCC was very efficient using the copper/coordination compound/peroxide system. After the treatment,some hydroxyl and carboxyl groups were increased and some low molecule compounds as syingaldehyde and vanillin were produced. The results could prove that the oxidation of side chain led to the cleavage of the linkage between the units of lignin substructure.

关 键 词：酶降解 低分子化合物 木素 红外光谱分析 吡啶 开环 侧链 马尾松 碳水化合物 香草醛 

分 类 号：TS79[轻工技术与工程—制浆造纸工程] S791.248[农业科学—林木遗传育种]