应用酶联—紫外连续监测法进行丙氨酸转氨酶活力测定  

Alanine Aminotransferase Assay with Contineous Enzyme Linked Ultraviolet Mornitor

作　　者：刘兴武[1] 李晶文[1] 王跃辉[1] 

机构地区：[1]齐齐哈尔医学院第二附属医院

出　　处：《黑龙江医学》1999年第1期7-8,共2页Heilongjiang Medical Journal

摘　　要：血清丙氨酸转氨酶(aLanine aminotrarshrase)的活力测定．对于肝脏病诊断及病情观察均有一定意义。我们自1992年应用酶促反应动力学的有关理论，以酶联—紫外连续监测法（即速率法)测定丙氨酸转酶得到了满意的效果。该法具有快速、结果准确、重复性好等优点。是目前较理想的测定方法。现介绍如下。This paper had measured ALT assay with contineous enzyme linked ultravioletmornitor and repeatcd nieasuring 40 times to definite value serum. The results were that X= 51. 3, VIS<80 without obvious dislurbancc and linear range was 5. O~ 300 u/l. It indicated this method was an ideal method to takc ALT assay rapidly, repeatedly and accuratelywith little disturbed factor in and out abroad.

关 键 词：连续监测法 血清丙氨酸转氨酶 诊断 肝脏病 病情观察 速率法 效果 酶活力测定 酶促反应动力学 紫外 

分 类 号：R446[医药卫生—诊断学] R575[医药卫生—临床医学]