大麦成熟胚胚性悬浮细胞系的建立及植株再生  

Establisbment of embryogenic cell suspension cultures from mature embryos of barley (Houdeum vuglare L.) and plantlet regeneration:

作　　者：项友斌[1] 刘静[1] 阮义理[2] 袁爱敏 

机构地区：[1]浙江省农业科学院生物技术中心,杭州310021 [2]浙江省农业科学院病毒实验室,杭州310021

出　　处：《浙江农业学报》1993年第4期213-219,共7页Acta Agriculturae Zhejiangensis

摘　　要：利用10个大麦品种成熟胚,在MS培养基上诱导愈伤组织,挑选不同培养时期的胚性愈伤组织在不同的液体培养基中进行悬浮培养。结果表明,愈伤组织继代培养时期和悬浮培养基对细胞系的建立有很大影响,一般以继代培养80-100天为最适;液体培养基以AA和MS较好。在AA培养基中加入AgNO_3Na_2S_2O_3可显著促进细胞生长和阻止愈伤组织长根和长芽或变褐死亡。悬浮培养过程中,细胞生长可分为三个时期,各时期细胞生长表现不同的特性,需采取不同的转培处理措施。经10-17周悬浮培养,在5个基因型中成功地建成了8个细胞团小、呈黄色或淡黄色、胞质浓、生长快的悬浮细胞系。植板后,除了2个再生白化苗,1个未能再生外,其余5个细胞系均再生了绿苗。Embryogenic calli were induced from the mature embryos of various barley genotypes. Cell suspension cultures were initated from the calli of incubated in liquid medium and eight fast-growing homogeneous cell suspension lines were established in five genotypes (cv. Zaosu 3, Supi 1, Yong 257, Interbell and 83055).Five of these cell suspensions regenerated green plantlets with different ability. For other three of these cell suspensions,only could two produced albino plantlets and one did not show morphogenic capacity. In the study, effect of liquid media and callus age on cell suspension culture were evaluated and the growth process of suspension cultures were investigated. The results indicated that AA and MS medium were relatively better than L3 and B5, and AgNO_3-Na_2S_2O_3 added in AA could greatly enhance calli growing, resulting in establishment of cell suspensions. Embryogenic calli by subculture for 80-100 days grew better with releasing mini-clusters (or cells) into liquid medium than the ones with subculture for shorter or longer period.

关 键 词：大麦 成熟胚 愈伤组织 植株再生 

分 类 号：S512.303.5[农业科学—作物学]