早孕期绒毛细胞短期培养的染色体脆性位点研究  

A study on chromosomal fragile sites of chorionic villus cell during early pregnancy

作　　者：孙永玉[1] 陶幼慈[1] 虎宝先[1] 蒋晓薇[1] 

机构地区：[1]同济医科大学附属协和医院妇产科遗传学室

出　　处：《中国优生与遗传杂志》1995年第1期19-22,共4页Chinese Journal of Birth Health & Heredity

摘　　要：筛选１０２份人工流产的绒毛标本，分组进行短期培养及染色体脆性位点（ＦＳ）的研究，结果表明：Ｍ１９９加ＭＴＸ（２组）（终浓度为４×１０～（－８）ｍｏ１／Ｌ）与单纯Ｍ１９９（１组）比较，染色体畸变率（ＣＡＲ）和脆性位点表达率（ＦＲ）差别均有显著意义（Ｐ＜０．０５）；Ｍ１９９加ＢｒｄＵ（终浓度为２×１０～（－４）ｍｏ１／Ｌ）（３组）与Ｍ１９９加ＭＴＸ比较ＦＲ差别有极显著意义（Ｐ＜０．０ｌ）；ＲＰＭＩ１６４０加ＭＴＸ（终浓度８×１０～（－８）ｍｏ１／Ｌ）（４组）与２组比较中期分裂相数差别有显著意义（Ｐ＜０．０５）。We studied fragile sites of 102 samples of chorionic villis obtained by artificial abortion using different medium and inductive model.It was found that:(1)There was significant difference(p<0.05) between M199 plus MTX and single M 199 in chromosomal aberration rate (CAR) and fragile site expression rate (FR); (2)There was a very significant difference(p<0. 01) between M199 plus BrdU and M199 plus MTX in FR;(3)There was a significant difference(p<0. 05) between RPMI 1640 plus MTX and M199 plus MTX in metaphase chromosomal numbers. Above results suggested RPMI 1640 is estremely suite for short term culture of chorionic villus cells.MTX and BrdU are ideal inducers for Chromosomal fragile sites of chorionic villus.

关 键 词：染色体脆性位点 MTX 绒毛细胞 早孕期 短期 人工流产 FS 培养 意义 差别 

分 类 号：R714[医药卫生—妇产科学] R730.231.3[医药卫生—临床医学]