聚合酶链反应检测疱疹Ⅱ型病毒先天性宫内感染的研究  

Study on the detection of Herpes Simplex Ⅱ DNA with the chorinic villi in early pregnancy by PCR technique

作　　者：周宁[1] 于萍[1] 陈诵芬[1] 徐增祥[1] 克丙申[1] 李红[1] 单乐媛[1] 尹格平[1] 

机构地区：[1]济南军区总医院妇产科,山东医科大学生物教研室

出　　处：《中国优生与遗传杂志》1995年第4期15-16,57,共3页Chinese Journal of Birth Health & Heredity

摘　　要：以人工合成的单纯疱症Ⅱ型病毒ＤＮＡ多聚基因片段为引物，采用聚合酶链反应技术（ＰＣＲ）检查早孕绒毛标本中疱疹Ⅱ型病毒感染，以８０例自发和人工流产的早孕绒毛组织标本进行基因扩增，３１例绒毛标本中检出ＨＳＶ－Ⅱ，并与同一绒毛标本的免疫组织化学结果进行比较，ＰＣＲ技术免除了昂贵繁杂的分子杂交过程，具有极高的敏感和特异性，为早期、快速产前诊断ＨＳＶ先天性宫内感染的最佳技术。Detection of Herpex simplex virus type-two DNA in villi samples from early pregnancy and sponetaneous abortion was performed by PCR.The results indicated that 31 of 80 cases detected HSV-ⅡDNA and its positive rate was increased by PCR conipairing with immunochemistry method.This method to study the HSV-2infection in clinical samples has been proved more sensitive,economic and simple.

关 键 词：先天性 标本 宫内感染 HSV 绒毛 早孕 疱疹 分子杂交 PCR) 人工合成 

分 类 号：R714[医药卫生—妇产科学] R446[医药卫生—临床医学]