大肠杆菌多核苷酸磷酸化酶的快速高效纯化  

RAPID AND EFFICIENT PURIFICATION OF POLYNUCLEOTIDE PHOSPHORYLASE FROM ESCHERICHIA COLI

作　　者：陈枢青[1] 韩晓旭[1] 王震熙[1] 

机构地区：[1]浙江医科大学生物化学教研室

出　　处：《浙江医科大学学报》1993年第1期8-11,共4页

摘　　要：利用快速蛋白质液相层析仪从大肠杆菌纯化到2500倍的多核苷酸磷酸化酶,回收率45.5％,SDS-聚丙烯酰胺凝胶电泳结果显示为单一组分,经测定未检出RNA酶、磷酸单酯酶和5’-核苷酸酶活性。Polynucleotide phosphorylase (PNPase)with a specific activity of 2500-fold higher than that of the original extract was obtained by Fast Protein Liquid Chromatography (FPLC)from E. coli, the re- covery rate being 45.5％. It proved to be of only one band on SDS-PAGE and no activities of RNase, phosphomonosterase and 5'-nucleotidase were detected.

关 键 词：大肠杆菌 多核苷酸 磷酸化酶 

分 类 号：R378.21[医药卫生—病原生物学]