快速ELISA、快速斑点ELISA和IHA检测华支睾吸虫病效果的比较  

Comparison of Fast-ELISA, Fast-Dos-ELISA and IHA in Detection of Clonorchiasis

作　　者：史小楚[1] 崔惠儿[1] 阮彩文[1] 周洁娴[1] 

机构地区：[1]广东省寄生虫病防治研究所,广州510300

出　　处：《广东卫生防疫》1997年第3期7-9,共3页Guangdong Journal of Health and Epidemic Prevention

摘　　要：采用快速ELISA、快速斑点ELISA和间接血凝试验（IHA）三种方法平行检测经粪便检查虫卵阳性的华支睾吸虫病人血清152份。结果快速ELISA的敏感性为98．68％，快速斑点ELISA为对，13％，IHA为63.16％，经比较敏感性之间差异均有显著性。以三种方法检测不同感染度的病人血清时，轻度感染者（EPG＜500）以快速ELISA最敏感（敏感性为98．89％）；以三种方法同时检测非流行区健康人血清106份，结果快速ELISA的特异性为97．17％，快速斑点ELISA为94．34％，IHA为92.45％，但三种方法比较特异性差异无显著性。因此，在临床诊断和大规模的流行病学调查中，最好选用快速ELISA。Fast - ELISA, Fast - Dot - ELISA and IHA were simultoneously applied to detect antibody in 152 serum samples from patients with clonorchiasis eggs posihve. The findingr showed the sensitivihes were 98.68%, 91. 13%, and 63.16% respechvely with significant difference. When three methods were applied to deteet sera with different clonorchiasis infection level, it was found that Fast - ELISA was a method of the most sensihve(sensihvity was 98. 89% ) in pahents with light in fection (EPG < 500). In testing 106 serum samples from normal individuals in nonepidemic area, the specificihes of Fast - ELISA Fast - Dot - ELISA and IHA were 97- 17%, 94.34% and 92.45% respechvely without significant difference. Therefore, Fast - ELISA was prefer to be used in clinical diagnosis and epidemiology investigation.

关 键 词：ELISA IHA 华支睾吸虫病 人血清 病效 病人 敏感性 显著性 平行 差异 

分 类 号：R446[医药卫生—诊断学] R532.23[医药卫生—临床医学]