羚蝎胶囊对实验性脑出血大鼠脑细胞的保护作用  

Protective Mechanism of Ling-xie Capsule for Cerebral Neural Cells Using Cerebral Hemorrhage Model in Rats

作　　者：吕嵘[1] 徐敏华[2] 韩志芬[1] 朱晓梅[1] 卫洪昌[1] 

机构地区：[1]上海中医药大学,上海200032 [2]上海市第二人民医院,上海200011

出　　处：《中国中医基础医学杂志》2002年第4期26-28,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：上海市科学技术发展基金 (No .9840 190 40 )

摘　　要：目的 :探讨羚蝎胶囊对实验性脑出血大鼠脑细胞的保护作用。方法 :采用胶原酶与肝素复合法制造大鼠脑出血模型 ,通过Fura 2双波长荧光法、化学法、高效液相色谱法 ,观察羚蝎胶囊对脑出血模型大鼠的脑细胞内Ca2 + 浓度、脑组织超氧化物歧化酶 (SOD)、丙二醛 (MDA)含量以及海马组织中兴奋性氨基酸 (EAA)含量的影响。结果 :羚蝎胶囊可以降低细胞内Ca2 + 的浓度 ,提高SOD的活力 ,减少MDA的生成 ,并且抑制海马组织中EAA的神经毒作用 (P <0 0 5 )。结论Objective To discover protective mechanism of Ling xie capsule in improving secondary neural injury using cerebral hemorrhages model in rat.Methods:Collagen and heparin combinations compound were injected into rat's nuclei caudate to establish a model of acute cerebral hemorrhage in rat.Intracellular free calciumion (Ca 2+ ) i were measured using Fura 2/AM fluoremetry technique,and the contents of SOD,MDA in brain tissue using chemical method,and the content of EAA in hippocampus tissue using HPLC,respectively.Result:We found out Ling xie capsule could decrease the concenrearion of (Ca 2+ ) i in injured neural cells,and increase the activity of SOD and reduce the production of MDA in brain tissue,and inhibit the increasing of the content of EAA in hippocampus tissue.These effects were significant analysis statistically ( P<0 05 ).Conclusion:Ling xie capsule was possessed of some productive effect for the rat's cerebral hemorrhage.

关 键 词：脑出血 羚蝎胶囊 细胞内CA^2+ SOD MDA EAA 

分 类 号：R289.1[医药卫生—方剂学]