利用根尖体细胞染色体鉴定种子类中药的研究  

Study of Identifying Seed Traditional Chinese Medicine by Somatic Cell Chromosome in Root-Tip

作　　者：聂凤禔[1] 李连怀[1] 董兰凤[1] 翟力东[1] 聂磊[1] 

机构地区：[1]河北医科大学药学院生药教研室,河北石家庄050017

出　　处：《中国中医基础医学杂志》2002年第4期76-78,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：河北省自然科学基金资助项目 (No .3 982 96)

摘　　要：目的 :揭示种子类中药染色体鉴定特征。方法 :种子 2 5℃萌发 ,根尖长 0 5cm切取 ,0 0 0 2mol/L 8-羟基喹啉的 0 0 3%秋水仙素液预处理 8h ,卡诺固定液固定 12h～ 2 4h ,1mol LHCl6 0℃解离 8min～ 12min ,卡宝液中压片 ,染色。镜检取分散良好的标本 ,冰冻揭盖片 ,中性树胶封片。显微照像、计数、测量。结果 :真伪品根尖体细胞染色体数目、组型有明显差异。结论 :种子类中药显微鉴定渗透应用细胞学理论方法 ,能反应不同物种外在性状差异的内在本质 ,准确鉴别真伪。Objective:To investigate the identificated characteristics of somatic cell chromosome in seed traditional Chinese medicine.Methods:Seeds were sprouted at 25℃,root tip of 0 5cm length were cut and treated in 0 03% colchicine diluted with 0 002mol/L 8 hydroxyquinoline for 8hours,fixing them for 12～14hours with Carnoy's fixative,differentiating for 8～12min with 1mol/L hydrochloric acid at 60℃,pressing to section in carmine for staining.Observing them under microscope,then choosing the samples that differentiated well,giving off the cover at frozen station and mount in Canada balsam(neutral mounting medium),micro photographing,counting and measuring.Results:There were significant differences in the numbers and karyotypes of somatic cell chromosomes in root tip between true and false ones.Conclusion:It could reflect inner essence from external morphological differences in different species and identify true or false exactly,to applying cytology theory and method to microscopically identifying seed traditional Chinese medicine.

关 键 词：根尖体细胞 种子 染色体 鉴定 

分 类 号：R931.5[医药卫生—生药学]