D1/D2/Cyt b-559几个不同寿命组分的荧光发射光谱的分辨  

RESOLUTION OF FLUORESCENCE EMISSION SPECTRA WITH VARIOUS LIFETIME COMPONENTS OF Dl/D2/cyt b-559

作　　者：于振宝[1] 匡廷云[1] 李成勇[2] 唐崇钦[1] 彭德川[1] 汤佩松[1] 

机构地区：[1]中国科学院植物研究所光合室,北京 100044 [2]中国科学院动物研究所生物膜和膜工程国家重点实验室,北京 100080

出　　处：《Acta Botanica Sinica》1993年第10期739-745,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家八五攀登计划;中国科学院重点科研项目资助

摘　　要：菠菜叶绿体光系统Ⅱ反应中心Ｄ１／Ｄ２／Ｃｙｔ ｂ－５５９复合物的荧光衰减包括４个组分，其寿命分别大约为１．０ｎｓ，５．９ｎｓ，２４ｎｓ和７３ｎｓ，所占整个荧光的量子产率依次为０．０５，０．３４，０．３５和０．２６。这些不同寿命组分的荧光发射光谱相互重叠在一起，稳态光谱只显示１个发射峰。根据相调荧光测定的“硬件”分析方法，通过选择检测器的相角，可以选择性地把各个寿命组分的荧光分别滤掉。如果５．PS Ⅱ reaction center D1/D2/Cyt b-559 purified from chloroplasts of spinach has four components of fluorescence decaying with lifetimes of 1. 0 ns,5. 9 ns,24 ns,and 73 ns whose fractions to total fluorescence yield are 0. 05, 0. 34,0. 35 and 0. 26 respectively. The fluorescence emission spectra of these lifetime components are closely overlapping, and only one peak is shown in steady state emission spectrum. Based on the hardware analysis of phase fluorometry ,by selection of the detector phase angle,the emission from various components could be individually suppressed. If the 5. 9 ns component was suppressed, the emission spectrum was red-shifted. On the contrary,the emission spectrum was blue-shifted when 73, ns component was suppressed. Based on the software analysis, the individual emission spectra were resolved with three lifetime components by measuring phase and modulation data at various wavelength. Compared with steady state spectrum, the emission maximum wavelength of 5. 9 ns component was blue-shifted from 682 nm to 680 nm,but those of 24 ns and 73 ns components were red-shifted to 685 nm and 683 nm respectively.

关 键 词：菠菜 反应中心 光合系统 荧光光谱 

分 类 号：S636.101[农业科学—蔬菜学]