Ca对小麦幼苗降低盐害效应的研究  

INFLUENCE OF CALCIUM ON ALLEVIATING NaCl-INDUCED INJURY EFFECTS IN WHEAT SEEDLINGS

作　　者：赵可夫[1] 卢元芳[2] 张宝泽[1] 衣建龙[1] 

机构地区：[1]山东师范大学生物系,济南250014 [2]曲阜师范大学生物系,曲阜273165

出　　处：《Acta Botanica Sinica》1993年第1期51-56,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：将三叶期小麦(Triticum aestivum,“预源1号”)移栽在含100mmol/L NaCl以及不同浓度CaCl的1/2强度Hoagland溶液中,另加适量的聚乙二醇(PEG)使其等渗,进行通气培养。实验结果表明,100mmol/L NaCl处理的小麦幼苗,质膜透性和过氧化作用增大,膜脂中的单半乳糖二甘油酯(MGDG)和双半乳糖二甘油酯(DGDG)增加和磷脂酰乙醇胺(PE)、磷脂酰胆碱(PC)等减少,经不同浓度的Na^+和 Ca^+之比处理,上述效应均呈下降趋势,Na^+/Ca^(2+)=10时,对盐效应的降低作用最大。通过自由基清除剂和SOD抑制剂试验,证明盐害效应与膜脂过氧化水平提高以及膜脂和膜脂肪酸组分变化有关,同时证明,Ca^(2+)对盐害效应的降低作用也与其有关。Wheat seedlings (Tritium aestivum 'No. 1 Yuyuan') with 3 leaves were transplanted to 1/2 strangth Hoagland nutrient solution containing 100 mmol/L NaC1 and supplemented with different concentrations of CaC1_2, which were mode isosmotic by adding polyethylene glycol (PEG) and aerated by pump. Results showed that the Na^+ content of shoots and roots, relative leaf expansion rate, plasrna membrane permeability, the levels of membrane lipid superoxida- tion and the molar percentage of monogalactosyl diglyceride(MGDG), digalactosyl diglyceri- de(DGDG) phosphatidyl choline(PC) and phosphatidyl ethanolamine(PE) in membrane lipids of roots increased, the plant dry weight, K^+ content, SOD activity and the molar percentage of phosphatidic acid(PA), phospatidyl inositol(PI), phosphatidyl glycerol(PG) and polyphospho- glyceric acid(PPG) decreased in roots. There was no change in sulfolipid(SL). However, the above mentioned salt injury effects were all alleviated by the different Na^+/Ca^(2+) ratios. The maximum alleviation of salt injury effect was at Na^+/Ca^(2+) ratio of 10. As three kinds of free radical scavengers were used to pretreat wheat seedlings prior to NaCl treatment the malon- dialdehyde(MDA) content decreased unanimously, but increased with SOD inhibitor sodium diethyldithiocarbamate (DDTC) prelreatment to wheat seedlings. Obviously, the salt injury ef- fects induced by NaC1 was relatied to the extent of superoxidation of membrane lipids and also to the composition of membrane of Ca^(2+) on lipids including their fatty acids as well. On the other hand, the alleviating effect of Ca(2+) on NaC1 induced injury in wheat seedlings was also in relation to them.

关 键 词：小麦 幼苗 抗盐性 钙 

分 类 号：S512.101[农业科学—作物学]