白香草木樨游离培养细胞高频率再生植株的激素调控  

HORMONE REGULATION IN HIGH-FREQUENCY PLANT REGENERATION FROM ISOLATED CULTURE CELL OF MELILOTUS ALBUS

作　　者：李风霞[1] 安利佳[1] 张俊敏[1] 罗希明[1] 何孟元 郝水[1] 

机构地区：[1]东北师范大学生物系,长春130024

出　　处：《Acta Botanica Sinica》1993年第5期374-378,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：从白香草木樨(Melilotus albus Desr.)胚轴外植体诱导愈伤组织。将已分化芽的愈伤组织转入含有不同浓度2,4-D(1—4mg/L)和6BA(0.2—8mg/L)的Ax培养基中进行液体振荡培养,得到细胞密度不同的9个细胞系。将细胞密度较大的4个细胞系置于含有不同浓度2,4-D(0.5—2mg/L)和6BA(0.1—2mg/L)的Ay_2培养基中液体浅层培养,只有L_5细胞系可再生愈伤组织。将愈伤组织转入含有0.2mg/L 2,4-D的Af_1培养基中,可分化出大量的幼芽。将幼芽转入去掉2,4-D,另外附加1mg/L赤霉素的Af_2培养基中,幼芽继续发育成幼苗。在0.5—1mg/L2,4-D和0.7mg/L6BA条件形成的单细胞愈伤组织,具有高频率再生植株(793.3％—1127％)的能力。较低浓度的2,4-D可以明显提高单细胞愈伤组织的分化频率。Calli were induced from plumular axis of Melilotus albus. Calli of differentiated buds were inoculated in liquid Ax media containing 2, 4-D(1—4mg/L)and 6BA(0.2—8mg/L)for vibrat- ing culture. 9 cell lines were obtained among which 4 were cultured in Ay2 media containing 2, 4-D (0.5—2mg/L) and 6BA (0.1—2mg/L) and only cell line, L_5 formed calli. The calli were transferred onto Af_1 medium containing 0.2 mg/L 2, 4-D,where many young buds were differ- entiated,the buds were then transferred onto Af_2 medium (Af_1 -- 2, 4--D+1mg/L GA_3), where they developed into young seedlings continuously. High regeneration frequency (793.3%— 1127%) of calli were obtained from isolated cells cultured in 0.5—1mg/L2, 4-D, 0.7mg/L 6BA. Differentiation frequency of calli from isolated culture cells was increased on the medium contuining lower concentration of 2, 4-D.

关 键 词：白香草木樨 细胞培养 植株再生 

分 类 号：Q949.751.9[生物学—植物学]