抗除草剂基因和荧光素酶基因在小麦愈伤组织中的稳定表达  

STABILITY EXPRESSION OF HERBICIDE RESISTANT GENE AND LUCIFERASE GENE IN CALLI DERIVED FROM WHEAT PROTOPLASTS

作　　者：杨书礼 曾君祉[2] 吴有强[2] 朱小平[2] 王东江[2] 张健[2] 

机构地区：[1]北京大学生物系蛋白质工程植物基因工程国家重点实验室,北京 100871 [2]中国科学院遗传研究所,北京 100101

出　　处：《Acta Botanica Sinica》1993年第11期825-830,共6页Acta Botanica Sinica（植物学报：英文版）

摘　　要：用ＰＥＧ介导法将抗除草剂基因和萤火虫荧光素酶基因转入小麦原生质体，在含５０ｍｇ／Ｌ Ｂａｓｔａ的培养基上筛选出有抗性的愈伤组织。ＤＮＡ分子杂交结果表明ｂａｒ基因和ｌｕｃ基因都已整合到小麦细胞基因组中，萤火虫荧光素酶活性测定也检测到ｌｕｃ基因的表达。Plasmid pJIT101 containing bar gene and luc gene was transferred into wheat protoplasts mediated with PEG 1450. Transformants were then selected out in medium containing 50 mg/L Basta, a phosphinothricin (PPT)containing chemical product. The results of DNA hybridizaion indicated that both bar gene and luc gene had integerated into transformant genome. The luciferase activity has also been detected in those transformants.

关 键 词：小麦 荧光素酶 除草剂 基因 

分 类 号：S188[农业科学—农业基础科学]