日照强度对观叶菠萝叶绿体内含物变化的影响  

EFFECT OF SUN LIGHT INTENSITY ON THE CHANGE OF CONTENTS IN THE CHLOROPLASTS OF TILLANDSIA USNECIAES

作　　者：吴敦肃 陈一新[1] 李瑞秋 高小彦[1] 郭一松[1] 

机构地区：[1]中国科学院上海植物生理研究所

出　　处：《Acta Botanica Sinica》1993年第12期906-910,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：用在强光下固定观叶菠萝自顶端至基部６层叶片的方法，发现第１层至第６层叶片的叶绿体内均有一种球形物质，用常规电镜的双染色法不能使之着色，其随阳光强弱有明显的日变化，即８：００尚未出现，１０：００出现少数，１２：００大量出现，１４：００数量大为减少，１６：００消失。基部老叶片内球形物和淀粉颗粒极少，用四甲亚胺高碘酸银法染球形物成黑色，证明为复合碳水化合物。After the leaves of Tillandsia usneciaes were fixed in 2-5% glutaraldehyde under strong sun light,some globular objects were found in the chloroplast. These globular objects did not take the ordinary U-Pb double stain but they showed a cyclic change in amount following the change of sum light intensity. At 8 O'clock in the morning,only a few globular objects appeared in the chloroplast, At 10 : 00 a. m. ,there were several globular objects and they greatly increased at noon and gradually reduced in the afternoon, and finally disappearred at 4 : 00 p. m. For detection of periodie acid-reactive complex carbohydrates with silver methenamine staining technique , the globular objects stained black. This result demonstrated that the globular objects may be polymers of in-termediat products of the carbon cycle in photosynthesis and C_3 compounds in malate de-carboxylation.

关 键 词：观叶菠萝 叶绿体 球形物 日照强度 

分 类 号：Q949.718.1[生物学—植物学]