益心口服液药物预处理对大鼠心肌缺血再灌注损伤的影响  

Pharmacological preconditioning of Yixin Oral Liquid (YOL) during myocardium ischemia and reperfusion in rat

作　　者：卫洪昌[1] 王秀薇[2] 吕嵘[1] 程金波[1] 朱晓梅[1] 吴倩[3] 章忱[1] 

机构地区：[1]上海中医药大学病理教研室,上海200032 [2]上海中医药大学附属龙华医院,上海200032 [3]上海中医药大学测试中心,上海200032

出　　处：《药学实践杂志》2001年第3期157-159,共3页Journal of Pharmaceutical Practice

基　　金：上海市卫生局课题 (98Z0 15 ) ;上海市科委课题(99ZB14 0 45 )

摘　　要：目的 :研究益心口服液药物预处理对大鼠心肌缺血再灌注损伤的保护作用。方法 :设正常、假手术、缺血再灌注、经典缺血预处理及益心口服液组 ,观察各组大鼠的血流动力学以及心肌细胞内钙离子浓度变化。结果 :经典预处理组、益心口服液组再灌注 60min后LVSP、±dp/dt均显著高于缺血再灌组 (P <0 .0 5) ;预处理组再灌注 60min后LVSP、±dp/dt均显著高于益心口服液组 (P <0 .0 5)。经典预处理组、益心口服液组钙离子浓度低于缺血再灌注组 (P <0 .0 5) ,益心口服液组钙离子浓度高于经典预处理组 (P <0 .0 5)。结论 :益心口服液预处理对大鼠心肌缺血再灌注损伤具有一定保护作用 ,部分机制可能是减轻心肌细胞内钙离子超载所带来的损害。OBJECTIVE:To investigate the protective mechanism of YOL on ischemia/reperfusion (I/R) myocardium.METHODS:48 male SD rats,weighting (260±30)g,were divided into 6 groups.They were normal control,I/R,classical ischemic precondition (C-IPC),sham-operated,Isosorbide dinitrate and YOL.The intracellular calcium concentration of the cardiomyocyte and hemodynamic changes were measured in all groups.RESULTS: LVSP、±dp/dt in C-IPC and YOL tread with ischemia 30 min and followed by reperfusion 60 min were obviously higher in I/R( P <0.05) while LVSP、±dp/dt of C-IPC was alsodefinitely high compared with YOL( P <0.05).The intracellular calcium concentration in C-IPC and YOL were lower in I/R( P <0.05), while [Ca 2+ ]I of YOL was increased in C-IPC ( P <0.05).CONCLUSIONS:Yixin oral Liquid(YOL) preconditioning can protect the myocardium from I/R. The possible protective mechanism may be due to decrease calcium overload in cardiomyocyte.

关 键 词：益心口服液 血流动力学 心肌缺血 再灌注损伤 大鼠 药物预处理 

分 类 号：R259.422[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]