两株蓖麻蚕核型多角体病毒DNA同源性的研究  

Studies on DNA Homology between Two Nuclear Polyhedrosis Viruses from Attacus ricini Larvae

作　　者：严银钫[1] 罗经[1] 

机构地区：[1]中国科学院武汉病毒研究所

出　　处：《中国病毒学》1993年第3期257-262,共6页Virologica Sinica

基　　金：国家自然科学基金资助项目之一

摘　　要：两株不同来源的蓖麻蚕核型多角体病毒(ArscsNPV和ArNPV)经提纯后,使用SDS—苯酚抽提病毒核酸,并使用限制性内切酶EcoRI,BamHI酶解后,用分子杂交方法与缺口平移标记的ArscsNPV-DNA探针杂交,分析了两株蓖麻蚕NPV病毒核酸的同源性。EcoRI酶解的ArNPV-DNA产生8个片段,其中5个片段能与ArscsNPV-DNA探针杂交。BamHI酶解ArNPV-DNA产生7个片段,其中6个片段能与ArscsNPV-DNA探针杂交。结果表明:两株蓖麻蚕NPV之间病毒核酸具有很高的同源性。使用斑点杂交方法分析了ArscsNPV与ArNPV,柞蚕NPV及家蚕NPV之间的核酸同源性,结果表明:ArscsNPV与ArNPV,柞蚕NPV具有同源性。而与家蚕NPV无核酸同源性。Arscs NPV DNA and Ar NPV DNA purified by SDS-phenol method were digested by restriction endonucleases EcoRI and BamHI. Arscs NPV DNA resulted in 5,5 fragments and Ar NPA DNA result ed in 8,7 fragments respectively after analysis on agarose gel electrophoresis. Five of eight fragments of Ar NPV DNA digested by EcoRI could be hybridized with probe of Arscs NPV DNA labeled by ra- dioactive isotope ^(32)p,and six of seven fragments of Ar NPV DNA digested by BamH I could be hy- bridized with probe. Dot blot hybridization showed that there was homology between Arscs NPV, Ar NPV,Ap NPV but no homology between Arscs NPV and Bm NPV.

关 键 词：蓖麻蚕 核多角体病毒 同源性 DNA 

分 类 号：S884.59[农业科学—特种经济动物饲养]