兔中性粒细胞防御素对HL-60细胞毒性及对病毒的直接灭活作用  

Cytotoxicity to HL-60 cell line and direct viral inactivation of neutrophil defensins

作　　者：吴琦[1] 徐罗玲 李成梁[1] 王伯瑶[1] 郑国瑛[2] 陈立礼[2] 

机构地区：[1]华西医科大学病理生理学教研室,成都610041 [2]四川省卫生防疫站

出　　处：《中国病理生理杂志》1993年第6期754-757,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：本研究从新西兰家兔腹腔渗出中性粒细胞溶酶体内提取酸溶性组分,经制备性AU-PAGE获得纯化的防御素(NP_1和NP_2),SDS-PAGE测定分子量为3kd左右。以HL-60细胞作靶细胞,当NP_1或NP_2在100μg/ml浓度时,可杀死60%以上的HL-60细胞。NP_1(26μg/ml)还可直接灭活单纯疱疹病毒Ⅰ型和乙型流感病毒,分别使病毒感染滴度降度98.2%和90%。本研究结果提示,中性粒细胞可合成和分泌肿瘤细胞毒性和抗病毒活性肽。A number of low molecular weight cationic peptides, named defensins,were purified from neutrophil granuals. Its in vitro cytotoxicity to HL-60 cell line anddirect viral inactivation were examined. The results showed that when incubated with NP-1 and NP-2(100μg/ml) fot 6 hours, more than 60% of HL-60 cells were killed. WhenHerpes simplex virus type I(HSy-I) and influenza virus B strain Beijing 87-1 wereincubated with NP-1(260μg/ml) at 37°C in water bath for 60 min, a marked reduction(98.2% and 90% ) in virus TCID_50 was observed. The results suggested that neutrophilgranulocyte might be able to synthesize peptides which have the cytotoxic and antiviralacitivities.

关 键 词：嗜中性 白细胞 细胞毒性 免疫学 

分 类 号：R392.12[医药卫生—免疫学]