缺氧对培养的猪肺动脉内皮细胞胞浆游离钙的影响  

Hypoxia increases intracellular free calcium in endothelial cells of porcine pulmonary artery

作　　者：胡清华[1] 金咸瑢[1] 王迪浔[1] 

机构地区：[1]同济医科大学病理生理学教研室,武汉430030

出　　处：《中国病理生理杂志》1993年第6期690-693,共4页Chinese Journal of Pathophysiology

摘　　要：本文应用荧光探针Fura-2/AM测定胞浆游离钙浓度([Ca^(2+)]i)技术,观察培养的猪肺动脉内皮细胞[Ca^(2+)]i在缺氧时变化。实验发现:向细胞悬液中充氮气造成缺氧时,肺动脉内皮细胞[Ca^(2+)]i升高81±21%(P<0.05,n=8)。结果提示肺动脉内皮细胞钙信使系统可能参与缺氧所致血管反应。Alteration of intracellular free calcium during hypoxia was studied in cul-tured porcine pulmonary arterial endothelial cells. Intracellular free calcium was measuredwith the fluorescent prob, Fura-2/AM. It was found that hypoxia induced by bubbling N_2 to cells suspension increased intracellular free calcium of endothelial cells by 81±21%(n=8, P<0.05). These changes of free calcium might play an important role inpulmonary vascular response to hypoxia.

关 键 词：钙 内皮 细胞 缺氧 

分 类 号：R594.3[医药卫生—内科学]