克山病病区粮饲养豚鼠的冠状动脉平滑肌细胞膜电位的研究  

Study on membrane potential of coronary artery smooth muscle of guinea pig fed with the grains from Keshan disease area

作　　者：孙岩[1] 王磊一[1] 刘志向[1] 柴象枢[1] 

机构地区：[1]山东省医学科学院生理研究室,济南250001

出　　处：《中国病理生理杂志》1993年第6期765-769,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：本文应用细胞内微电极方法,研究了克山病病区粮喂饲豚鼠的冠脉平滑肌细胞膜电位变化,取得如下结果:(1)实验动物冠脉平滑肌细胞静息膜电位为-49.80±5.3mV,对照组动物为-49.98±6.2mV(P>0.05)。(2)硒可使实验组动物冠脉平滑肌细胞膜电位去极化,其程度明显小于对照动物。(3)氯化钾和乙酰胆碱可分别引起实验动物冠脉平滑肌细胞膜电位去极化和超极化,且较对照动物的变化明显增大。这些结果提示:在低硒状态下,冠脉平滑肌的功能状态发生明显变化,使之对刺激的敏感性增加,因而认为血管因素在克山病病区粮饲养动物的心肌坏死灶产生过程中起着重要作用。The reactivity of vascular smooh muscle cell (VSMC) of coronary artery ofguinea pig fed with grains from Keshan disease area were studied by intracellular microelec-trode method. The glass micropipette of tip resistance 30-50 MΩ filled with 3 M KCl wasinserted from the inner surface of the vasclar segment. The resting membrane potential (Em)of VSMC of coronary artery was observed. The results were as follows: (1) Em ofVSMC of experimental group was -49.80±5.3mV. (2) The Se induced VSMC depolari-zation revealed a dose-response dependent manner in the experimental animal. The degreeof the depolarization was smaller than those in the control guinea pig. (3) KCl and AChcaused membrane depolarization and hyperpolarization respectively in the experimental group,but the changes of Em was higher than those in the control. From these facts it wasconcluded that the reactivity of coronary artery VSMC was increased in experimentalgroup. It was suggested that the vessel's factor play an important role in the pathogenesisof myocardial necrosis in experimental group animal.

关 键 词：克山病 冠状血管 平滑肌 膜电位 

分 类 号：R542.302[医药卫生—心血管疾病]