红豆草悬浮培养细胞原生质体再生植株  

PLANT REGENERATION FROM IN SUSPENSION CULTURE CELL PROTOPLAST OF SAINFOIN

作　　者：罗希明[1] 谢雪菊[1] 

机构地区：[1]东北师范大学生物系,长春130024

出　　处：《中国草地》1993年第1期63-67,共5页Grassland of China

摘　　要：切取生长7～10天无菌苗下胚轴,接种在改良SL培养基上形成愈伤组织,转入SL液体培养基上振荡培养,建立悬浮培养细胞。用E_5酶液(1.6％Cellulase+0.8％Pectolyase Y—23+0.2％Macerase+CPW9M)游离原生质体,用K8P和V—Km原生质体培养基浅层培养,不加液2个月后形成愈伤组织,愈伤组织经增殖后转入分化培养基M_3(MS无机盐+B_5有机成分+0.5mg/L ZT+0.5mg/L KT+0.5mg/L BA+0.2rag/L NAA)一个月后分化出芽,转入M_5(MS+0.7mg/L BA+0.2mg/L NAA)发育成苗,再转入M_7(1/2MS+0.2mg/L IBA)生根,再生完整植株。Hypocotyls from 7-10 day-old aseptic seedings of sainfoin (Onobrychis viciaefolia) were cut into strips, then,they were placed on solid SL medium to form calli. The calli were transferred into liquid SL medium for obtaining suspension culture cells. Protoplasts were isolated from the suspension culture cells in an enzyme solution containing 1.6% Cellulase R-10,0.8% Pectolyase y-23,0.2% Maeerase,CPW9M. The protoplasts were cultured in K8P and V-KM media to form eaili after two months. The calli were transferred onto. differentiation medium M3 containing MS mineral,B5 organ,0.5 mg/L ZT, 0.5mg/L KT, 0.5mg/LBA, 0.2kg/L NAA. Shoot formation was initiated on M3 medium,and the shoots were transferred onto M5 medium containing MS, 0.7mg/L BA, 0.2mg/L NAA to develop into plantlets, and then into perfect plants.

关 键 词：红豆草 原生质体 再生植株 

分 类 号：S541.403.5[农业科学—作物学]