抗病毒抗生素16704A的研究 Ⅱ.抗病毒活性的研究  

Studies on antiviral antibiotic 16704A Ⅱ. Antiviral activities of 16704A

作　　者：孙雪冰[1] 陶佩珍[1] 司书毅[1] 娄志贤[1] 姚恩泰[1] 姚天爵[1] 王淑琴[1] 

机构地区：[1]中国医学科学院中国协和医科大学医药生物技术研究所,北京100050

出　　处：《中国抗生素杂志》2001年第2期94-97,共4页Chinese Journal of Antibiotics

摘　　要：在常规细胞培养筛选中 ,我们发现一株由云南曲靖地区土壤分离的链霉菌的发酵液有广谱抗病毒活性 ,不抗细菌及真菌 ,经分离与纯化得到 16 70 4A纯品 ,结构鉴定为 Bafilomycin A1。16 70 4A在 Vero细胞内对单疱病毒 1型 ,2型及水疱性口炎病毒均有明显抑制作用 ,用 CPE方法 ,IC5 0 分别为 0 .6 5、0 .78及 0 .39mg/ L,选择指数分别为 14.7、12 .2及 2 4.4;用 PFU方法 ,IC5 0 分别为 0 .36、0 .47及 0 .11mg/ L,选择指数分别为 2 6 .5、2 0 .3及 86 .6。A strain of Streptomyces isolated from a soil sample collected in Qu Jing area of Yunnan province displayed broad spectrum of antiviral activities in our routine screening program. The fermentation broth of this strain ( Streptomyces 16704) did not show anti bacterial and anti fungal activities. After isolation, purification and identification, the antiviral antibiotic (16704A) was turned to identified with bafilomycin A1. In vero cells, 16704A showed anti herpes simplexvirus type 1, type 2 and vesicular stomatitis virus activities with IC 50 values as 0.65, 0.78 and 0.39mg/L, respectively by CPE method, with selective indices as 14.7, 12.2 and 24.4 respectively; with IC 50 values as 0.36, 0.47 and 0.11mg/L, respectively by PFU method, with selective indices as 26.5, 20.3 and 86.6 respectively.

关 键 词：抗病毒抗生素 16704A 抗病毒活性 

分 类 号：R978.7[医药卫生—药品]