单克隆抗体双位点夹心ELISA测定人血清载脂蛋白E的研究  

Quantification of Human Serum Apolipoprotein E by ELISA Using Anti—apo E Monoclonal Antibodies

作　　者：张东升[1] 夏辉明[1] 赵明耀[1] 张春艳[1] 刘淮生[1] 吕新跃[2] 夏富臻 

机构地区：[1]河南医科大学病理生理教研室 [2]中国人民武装警察部队医学专科学校

出　　处：《中国动脉硬化杂志》1993年第1期55-60,共6页Chinese Journal of Arteriosclerosis

摘　　要：利用自制的抗人载脂蛋白E(apoE)单克隆抗体,建立了单克隆抗体双位点夹心ELISA测定apoE的方法,并对该方法的特异性、准确度、精密度和灵敏度进行了评价。载脂蛋白AⅠ、AⅡ、B、CⅠ、CⅡ、CⅢ不干扰测定,检测下限10ng,回收率101.2％,批内变异系数CV=5.9％,批间CV=8.1％。并测定了1048例正常人和各种疾病患者血清apoE含量。Monoclonal antibodies agaist human apolipoprotein E were prepared. Double McAbs Sandwich ELISA has been developed to quantitate apoE concentration in human serum. This paper presents the details of analytic procedure, and the evaluation of its specificity, precison, accuracy and sensitivity. The McAbs reacted specifically with apoE, no cross reaction with apoA Ⅰ、A Ⅱ, B, C Ⅰ , C Ⅱ , C Ⅲ was observed. The assay could detect 10ng of apoE, average recovery was 101.6％, the cofficients of variation of the intra—assay and inter—assay were 5.9％ and 8.1％, respectively. The assay was used to quantitate apoE levels in normol persons and patients.

关 键 词：单克隆抗体 ELISA 载脂蛋白E 

分 类 号：R446.62[医药卫生—诊断学]