香烟烟雾冷凝物诱导体外培养细胞程序外DNA合成的试验研究  

Unscheduled DNA Synthesis(UDS) Induced by Cigarette Smoke Condensate in Cultured Human Epithelial Cells and Whole Blood Lymphocytes

作　　者：韩成龙[1] 汤朝武[1] 欧阳学[1] 李良寿[1] 蒋豫图[2] 张习坦[2] 

机构地区：[1]第四军医大学,西安710032 [2]军事医学科学院

出　　处：《中国公共卫生学报》1993年第6期343-344,共2页

摘　　要：采用程序外DNA合成试验方法检测香烟冷凝物对体外培养的人表皮细胞和人全血淋巴细胞DNA的损伤作用。结果表明,香烟冷凝物能引起培养的表皮细胞和淋巴细胞的DNA损伤;在一定的作用物剂量范围内表现出剂量效应关系。由于在细胞培养环境中未加S_9组分活化酶,提示冷凝物中含有不需代谢活化就能直接导致DNA损伤的有害物质。UDS tests with short-term cultured human epithelial cells and whole blood lymphocytes were established. In these tests, hydroxyurea was used to suppress half-preserved DNA dupli- cation and, after exposing to DNA-damaging agentfor 3~5hours,the incorporated 3~H-TdR in cell DNA was assayed by liquid scientillation counting set to reflect DNA damage and repairing ability of the cells. The effect of cigarette smoke condensate (CSC)on UDS was studied. The results showed that CSC could induce DNA damage in the cultured epithelial cells and lymphocytes and a significant dose-response relationship between CSC concentra- tion and DNA damage was found at certain dose range (10~ 150ug/ml). This study added another evidence for the causal relationship between cigarette smoking amd certain kinds of cancer.

关 键 词：香烟烟雾 冷凝物 DNA 合成 细胞 

分 类 号：R994.1[医药卫生—毒理学]