低能量激光对人胎肝造血细胞的增殖作用  

Proliferative Effect of Low Energy Laser on Human Fetal Liver Hematopoietic Cells

作　　者：欧阳淹[1] 董利[1] 陈明泉[1] 孙酬经[2] 汪涟明[2] 钱美华[2] 顾锡荣[3] 王红叶[3] 吴溯帆[3] 

机构地区：[1]浙江省医学科学院,杭州市310013 [2]浙江省人民医院血液科,杭州市310013 [3]浙江省人民医院激光科,杭州市310013

出　　处：《中国激光医学杂志》1993年第3期131-134,共4页Chinese Journal of Laser Medicine & Surgery

基　　金：国家自然科学基金

摘　　要：低能量激光对生物体具有刺激作用。经Ar^+激光波长514.5nm照射后的人胎肝造血细胞,可以在体外培养体系中分裂增殖,造血细胞集落明显增多。用激光照射产生集落刺激因子(CSF)的细胞,可以提高CSF的活性,在造血细胞体外培养体系中,使用该CSF,也能明显地提高造血细胞集落的形成率。用激光技术来处理造血细胞,可以成倍地提高细胞产率。这一技术不仅有理论意义而且有实用价值。The stimulating effect of low energy laser on proliferation of the CM-CFUc and theCSF activity were investigated by cultured human fotal liver hemalopoietic cells in vitro.The number of CM-CFUc of human fetal liver hematopoietic cells after irradiation by Ar^+ laser(514.5nm, 0.15J/cm^2) was increased significantly as compared mith that with 488 nm laserirradiation. On the contrary, when a larger laser energy dose was used, the number of GM-CFUc decreased. The laser efficiency changed from stimulation into inhibition when a energydose more than 2.4 J/cm^2 was used. The CSF was prepared by cultured umbilical cord cells.When these cells were treated by laser less than 1.0J/cm^2, a significantly increased CSFactivity upon human fetal liver hematopoietic cells was shown. Again, the CSF activitychanged from stimulation into inhibition when a larger laser energy dose was used.

关 键 词：激光 胎肝造血细胞 集落刺激因子 

分 类 号：R454.2[医药卫生—治疗学]