DNA探针诊断间日疟的进一步研究  

FURTHER REPORT ON APPLICATION OF DNA PROBE IN DIAGNOSIS OF VIVAX MALARIA INFECTION

作　　者：张龙兴[1] 柴建华[2] 詹斌[1] 冯晓平[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所,上海200025 [2]复旦大学遗传学研究所,上海200433

出　　处：《中国寄生虫学与寄生虫病杂志》1993年第2期86-88,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：对以间日疟原虫特异的0.24kb DNA探针用点杂交试验出现阳性反应的部分恶性疟患者血样和现场镜检阴性的发热病人血样用Southern blotting法加以甄别。结果显示,点杂交阳性反应者本试验亦呈阳性反应。证实在部分恶性疟患者及镜检阴性的发热病人中可能同时感染间日疟原虫。2份点杂交可疑阳性的上海献血员血样,本试验呈阴性。Southern blotting with a labeled and linearized pUC 19 DNA containing a specific fragment of 0. 24 kb DNA of Plasmodium vivax asexual blood stages (kindly offered by Dr. C. Kidson )was used for further identification of blood samples showing positive reaction by dot-blot hybridization. The results showed that those with positive reaction from patients with P. vivax, with P. falciparum or with fever but with negative microscopic findings were also positive by Southern blotting. It was confirmed that some of those positive with P. falciparum were likely to infect P. vivax at the same time. So did a part of those with fever but negative in the blood films (Figs. 1,2).

关 键 词：DNA探针 诊断 间日疟 

分 类 号：R531.304[医药卫生—内科学]