间接荧光抗体试验检测感染广州血管圆线虫大鼠血清抗体的观察  

DETECTION OF SERUM ANTIBODIES IN RATS INFECTED WITH ANGIOSTRONGYLUS CANTONENSIS BY INDIRECT FLUORESCENT ANTIBODY TEST

作　　者：梁浩昆[1] 晋雪香[2] 冯祖梅 王梅英[2] 潘松涛[1] 苏寿(氵氐) 

机构地区：[1]广州医学院寄生虫学教研室,广州510182 [2]河南医科大学寄生虫学教研室,郑州450052

出　　处：《中国寄生虫学与寄生虫病杂志》1993年第4期286-287,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：用广州血管圆线虫成虫切制成 4 μm厚的冰冻切片作抗原,用间接荧光抗体试验检测感染广州血管圆线虫大鼠血清的抗体,结果在感染后 2 wk的 50份鼠血清中,呈阳性反应46份,阳性率为 92.0％;在感染后 4 wk的 19份鼠血清中,呈阳性反应 19份,阳性率为 100％;对照的阴性血清及盐水均呈阴性反应。本法检出率高,且感染早期出现阳性反应,可作广州血管圆线虫病早期辅助诊断。Frozen section specimens with a thickness of 4 μm were made from adult worms of An-giostrongylus cantonensis in order to detect the serum antibodies in rats infected with An-giostrongylus cantonensis by indirect fluorescent antibody test (IFAT). The results showed that the positive rates of the serum samples 2 and 4 weeks after infection were 92. 0%(46/ 50) and l00% (19/19), respectively. The results of serum samples in healthy rats and normal saline (as control) were negative. This technique proved to have a highly detectable rate and the positive reaction might occur in early infection. It might be used for the early supplementary diagnosis of angiostrongyliasis.

关 键 词：血管圆线虫 荧光抗体试验 血清抗体 

分 类 号：R532.19[医药卫生—内科学]