Ⅱ型碳酸酐酶阻断剂抑制MGC体外骨吸收研究  

Effect of carbonic anhydrase Ⅱ inhibitor on bone resorption by multinucleated giant cells from giant cell tumour of bone

作　　者：赵宁侠[1] 于世凤[1] 庞淑珍[1] 

机构地区：[1]北京医科大学口腔医院病理室

出　　处：《中国骨质疏松杂志》1998年第2期24-26,共3页Chinese Journal of Osteoporosis

摘　　要：本文通过分离培养骨巨细胞瘤中的多核巨细胞与骨片共同培养，观察碳酸酐酶阻断剂乙酰唑胺（ａｃｅｔａｚｏｌａｍｉｄｅ）对骨吸收的影响。结果显示乙酰唑胺抑制多核巨细胞的骨吸收。浓度为１０－６Ｍ时骨吸收陷窝数和骨吸收面积均显著减少（Ｐ＜０．００１）。浓度为１０－８Ｍ时，只对骨吸收面积有显著抑制作用（Ｐ３天＜０．０５，Ｐ９天＜０．００１）。随时间延长，骨吸收陷窝及骨吸收面积呈显著增加（Ｐ＜０．００１）。结果表明乙酰唑胺对多核巨细胞骨吸收有抑制作用，说明多核巨细胞与破骨细胞在骨吸收机制方面的相似性。In this study we separated multinucleated giant cells MGC) from giant cell tumour of bone (GCT) and cultured them with bone slice.We observed the effect of acetazolamide,carbonic anhydrase (CA) Ⅱ inhibitor,on bone resorption by MGC.The result showed that acetazolamide inhibited the MGC bone resorption.Compared with the control,acetazolamide at the concentration of 10-6M could not only inhibit the pit numbers but also the areas of pits effectively (P<0001) and could compeletly inhibit the bone resorption.But acetazolamide at the concentraction of 10-8M could only inhibit the pit areas (on day 3 P<005,on day 9 P<0001).With time prolonged,the pit numbers and areas further increased (P<0001).It showed that CA Ⅱ inhibitor plays an important role in MGC bone resorption.It further demonstracted that MGC had the same mechanism in bone resorption as the osteoclasts.

关 键 词：骨吸收 多核巨细胞 乙酰唑胺 体外 抑制作用 碳酸酐酶 制方 共同培养 破骨细胞 骨巨细胞瘤 

分 类 号：R681[医药卫生—骨科学] R738.1[医药卫生—外科学]