三种大鼠骨质疏松模型的比较研究  

Comparison study on three osteoporotic rats models

作　　者：刘康[1] 季晖[1] 李绍平[1] 于冰[1] 李耐三[1] 

机构地区：[1]中国药科大学药理学教研室

出　　处：《中国骨质疏松杂志》1998年第4期13-18,共6页Chinese Journal of Osteoporosis

基　　金：江苏省科委应用基础基金

摘　　要：用维Ａ酸、摘除卵巢及糖皮质激素诱导大鼠骨质疏松症，以血清碱性磷酸酶（ｓ－ＡＬＰ），血清钙（ｓ－Ｃａ）、磷（ｓ－Ｐ），尿钙／肌酐（ｕ－Ｃａ／Ｃｒ）及羟脯氨酸／肌酐（ｕ－Ｈｏｐ／Ｃｒ）等为指标，引入骨表观线密度（Ｗ／Ｌ）、面密度（Ｗ／Ｌφ）两个新的骨指数，对该三种较常用的大鼠骨质疏松模型进行了比较研究。结果显示大剂量维Ａ酸组（９０、１００、１２０ｍｇ／ｋｇ）及摘除卵巢组骨重（Ｗ）减轻，Ｗ／Ｌ、Ｗ／Ｌφ明显降低（ｐ＜０．０１，ｐ＜０．０５），形成典型的大鼠骨质疏松症；糖皮质激素组ｓ－ＡＬＰ、ｕ－Ｃａ／Ｃｒ及ｕ－Ｈｏｐ／Ｃｒ显著升高（ｐ＜０．０１，ｐ＜０．０１），但Ｗ／Ｌ。Retinoic acid(RA),ovariectomy and glucocorticoid(GC)were used to induce osteoporosis in rats.The markers included serum alkaline phosphatase (s ALP),serum calcium(s Ca),and urine hydroxyproline/creatinine(u Hop/Cr).Bone apparent line density(W/L)and bone apparent area density(W/L φ)were regarded as two new bone indexes,which were also used to compare these three osteoporosis models in rats.The results indicated that higher doses of RA(90,100,120mg/kg)and ovariectomy could decrease bone mass,W/L and W/L φ apparently( p <0.01, p <0.05),which formed the two typical models.S ALP、u Ca/Cr and u Hop/Cr in rats treated with GC improved obviously( p <0.01, p <0.05),but W/L φ had no changes.

关 键 词：大鼠 维A酸 骨质疏松症 糖皮质激素 骨质疏松模型 摘除 卵巢 ALP 尿钙 大剂量 

分 类 号：R285.5[医药卫生—中药学] R681[医药卫生—中医学]