肝内结石患者肝内胆管及胆囊的粘液组化和凝集素受体研究  

Study on mucous histochemistry and lectin receptor of intrahepatic bile ducts and gallbladder in hepatolithiasis

作　　者：蓝祥海[1] 邓宗鍉 

机构地区：[1]四川沪州医学院附院普外科,646000 [2]广州孙逸仙纪念医院普外科

出　　处：《中华肝胆外科杂志》1998年第1期24-26,共3页Chinese Journal of Hepatobiliary Surgery

摘　　要：采用凝集素免疫组织化学及常规粘液组织化学方法对15例肝内结石病人及3例正常人肝脏的肝内胆管和胆囊的形态学及组织化学特性进行了研究。结果表明,肝内结石病人肝内胆管壁内粘液腺大量增生,浆液腺及胆管上皮出现粘液化改变;胆囊粘膜基本完整,上皮也有轻度粘液化;胆囊与肝内胆管的凝集素受体表达存在一致性,阳性范围与粘液组织化学粘液染色区相对应,且均以岩藻糖及半乳糖含量最丰富。故认为肝内结石主要形成于肝内,胆囊也主动参与了所伴胆囊结石的形成,胆囊的改变不足以支持常规胆囊切除。The morphology and histochemical features of intrahepatic bile ducts and gallbladder in 15 patients with hepatolithiasis and livers of 3 normal subjects were studied using lectin immunohistoche- mistry and conventional mucous histochemistry.The results disclosed that there were a large amounts of proliferated mucous glands inside the intrahepatic bile ductal wall in hepatolithiasis.Serous glands and intra- hepatic bile duct epithelia presented mucous metaplasia.The mucosa of gallbladder was intact on the whole and appeared slight mucous meraplastic change.The expression of lectin receptor in intrahepatic bile ducts and gallbladders showed no difference.The extent of lectin positive stainings was corresponding with those of mucous histochemistry stainings.The contents of fucose and mannose were more than other carbohy- drats.Therefore,it might be that intrahepatic calculi were mainly formed in intrahepatic bile ducts,while gallbladder participated actively in the formation of accompaning gallbladder stones.The morphological changes of gallbladder in hepatolithiasis were not enough to support performing conventional cholecystec- tomy.

关 键 词：肝内胆管 肝内结石 胆囊 凝集素受体 粘液腺 上皮 病人 表达 糖含量 增生 

分 类 号：R657[医药卫生—外科学] R575[医药卫生—临床医学]