正常人血清抗酒石酸盐酸性磷酸酶活性的观察  

Serum TRAP levels in normal human beings

作　　者：侯静[1] 蔡桂英[1] 杨霞[1] 赵玉华[1] 

机构地区：[1]华西医科大学生物化学教研室

出　　处：《中国骨质疏松杂志》1999年第1期43-45,共3页Chinese Journal of Osteoporosis

摘　　要：抗酒石酸盐酸性磷酸酶（Ｔａｒｔｒａｔｅ－ＲｅｓｉｓｔａｎｔＡｃｉｄＰｈｏｓｐｈａｔａｓｅ，ＴＲＡＰ）是破骨细胞功能的重要标志物，它的活性与破骨细胞活性呈正相关。ＴＲＡＰ由破骨细胞产生后分泌入血，因此可通过测定血清中ＴＲＡＰ的活性来了解破骨细胞活性。本文为观察ＴＲＡＰ活性在正常人血清中动态变化趋势，采用对硝基酚磷酸盐法测定了２３７例（年龄０．５～７２岁，平均年龄３５．３±１８．０）不同年龄段（每隔１０岁为一年龄段）正常人血清ＴＲＡＰ活性。结果显示：①２３７例正常人血清ＴＲＡＰ活性水平为９．６８±５．６５Ｕ／Ｌ，其中男性为９．８８±５．８０Ｕ／Ｌ（ｎ＝１２９），女性为９．４７±５．４０Ｕ／Ｌ（ｎ＝１０８）；②血清ＴＲＡＰ活性水平与年龄呈明显负相关（ｒ＝－０．３３７，Ｐ＜０．０１），儿童及青少年时期，血清ＴＲＡＰ活性都较高；成年（２０岁）后，随年龄增长，其活性逐渐降低，在４０～４９岁达最低值（７．２１±２．７８Ｕ／Ｌ）。此后，男女两性的血清ＴＲＡＰ活性的变化表现出不同的趋势，男性血清ＴＲＡＰ活性基本保持在一定水平，而女性血清ＴＲＡＰ活性则开始逐渐回升。结果说明：血清ＴＲＡＰ活性可作为骨代谢过程中破骨细胞功能的较灵?Tartrate resistant acid phosphatase (TRAP) is a functional marker of osteoclasts.Its activity is positively correlated with the activity of osteoclasts.TRAP can be produced by osteoclasts and secreted into the blood.So we can assess the activity of osteoclasts by determining the activity of TRAP in serum.In order to understand the change tendency of TRAP in serum of normal human beings,we systematically determined the serum levels of TRAP activity in a group of 237 normal persons aged 0 5 72 years divided into different decades,using P nitrophenyl phosphate disodium salt (pNPP) as substrate.The results wee as follows.1)The serum level of TRAP was 9.68±5.65U/L in normal subjects,9.88±5.80U/L in males ( n =129),and 9.47±5.40U/L in females ( n =108).2)The serum level of TRAP was negatively correlated with the age ( r =-0.337, P <0.01).It was high in childhood and adolescence.After 20 years of age,it decreased with age,and dropped to the lowest level (7.20±2.80U/L)in 40 49 years of age.Then it was different in sexes;the serum TRAP activity maintained a certain level in males,while it rise again in females.Our results showed that serum TRAP can be a sensitive marker for osteoclastic function in bone metabolism;it is of significance for clinic and basic research in bone metabolism.

关 键 词：TRAP 正常人 人血清 破骨细胞 活性 观察 骨代谢 男性 年龄 女性 

分 类 号：R446[医药卫生—诊断学] R737[医药卫生—临床医学]