萃取催化分光光度法测定微量元素铜  

Extraction-catalytic kinetic spectrophotometric determination of trace element copper

作　　者：赵兴茹[1] 张焕弟[1] 贾欣欣[2] 

机构地区：[1]河北职工医学院,071000 [2]河北农业大学院

出　　处：《河北职工医学院学报》1999年第2期11-12,共2页Journal of Hebei Medical College for Continuing Education

摘　　要：本文研究了在pH9.2—9.5氨性缓冲溶液中,Cu(Ⅱ)催化H_2O_2氧化溴酚蓝和丁基罗丹明B等色染料离子对褪色的新指示反应,建立了较为广泛的快速终止催化反应的新方法。本方法测定铜,检出限为0.02μg/L,5次测定结果相对标准偏差(RSD)<4％,线性范围为0.1～10μg/L。用于直肠癌组织中微量元素铜的测定,结果满意。In this paper an isochromatic dye ion-pairs extraction - catalytic spectrophotometric method for the determination of trace copper is studied. The method is based on the copper catalyzed oxidation of isochromatic dye ion-pairs formed by buthylrhodamine B and bromophenol blue by hydrogen peroxide in ammoniac medium. The reaction time, concentration of isochromatic dye ion - pairs in aqueous phase and degree of reaction are controlled by extraction equilibrium. The detection limit of this method is 0.02μg/L.The linear range is 0.1-10μg/L respectively. The method has been applied to the determination of trace copper in tumor tissue with satisfactory results.

关 键 词：癌组织中 直肠癌 微量元素 RSD 测定结果 催化分光光度法 离子对 相对标准偏差 Cu(Ⅱ) 催化反应 

分 类 号：R446[医药卫生—诊断学] O657[医药卫生—临床医学]