去势合成肽疫苗研究  

A STUDY ON CASTRATING PEPTIDE VACCINE

作　　者：刘公平[1] 刘胜江[1] 杜念兴[1] 徐为燕[1] 

机构地区：[1]南京农业大学兽医系

出　　处：《中国农业科学》1993年第6期77-85,共9页Scientia Agricultura Sinica

摘　　要：用碳化二亚胺法,将人工合成的促黄体素释放激素(LHRH)共价连接到牛血清白蛋白(BSA)上,合成LHRH-BSA半抗原載体复合物。用此人工复合抗原,加福氏佐剂,免疫2月龄公兔,与不免疫的对照兔一起,在同样条件下饲养,定期检查各兔性功能及性器官发育,并测定血清中LHRH抗体及睾酮水平。结果各试验兔随着血清中LHRH抗体的产生,性功能丧失,阴茎及睾丸萎缩,睾丸生精管管腔塌陷,管壁仅由1-2层排列松散的生精细胞组成,生精细胞水泡样变性,生精管中无精子产生;血清睾酮水平明显低于对照兔。成年公兔注射LHRH抗血清被动免疫后,血清LH和睾酮水平立即明显下降。研究证明,实验兔接种LHRH-BSA后所产生的LHRH抗体能中和内源性LHRH的生物学活性,抑制垂体促性腺素的合成和分泌,导致实验兔性器官及性腺发育严重受阻,达到了预期的免疫去势效应。Luteinizing hormone releasing hormone(LHRH) was covalently conjugated to bovine serum albumin(BSA) by means of soluble carbodiimide.The synthetic LHRH-BSA complex was used to vaccinate three groups of male New Zealand rabbits aged two months.Three rab bits in group Ⅰ received three inoculatioas at intervals of three weeks; three rabbits in group Ⅱ and four rabbits in group Ⅲ received four and five inoculations respectively, while ten rabbits uninoculated in group Ⅳ were kept as control. All of these animals had the same initial body weight and were bred under the same condition After the third inoculation rabbits with production of antibodies to LHRH showed no sexual behavior, reduction of testes weight and atrophy of seminiferous tubules in which no spermatuzoom was observed.Two adult male rabbits injected with 6 ml of LHRH antiserum revealed decline in LH and testosterone levels in their serum.The experiment demonstrated that the immune response induced by inoculation of LHRH-BSA complex in young male rabbits neutralized the biological activity of endogenous LHRH and inhibited the synthesis and release of pituitary LH, FSH and testosterone, resulting in blocking the development of sexual organs and gonads and elimilating the sexual ability.Therefore, LHRH-BSA complex is promising to be a vaccine for castration as an alternative to conventional surgical operation.

关 键 词：阉割 合成肽疫苗 

分 类 号：S857.129[农业科学—临床兽医学]