高效液相色谱法测定板蓝根中丁香酸兔体内药物动力学  

HPLC determination of pharmacokinetics of syringic acid from radix isatidis in rabbits

作　　者：刘云海[1] 汤杰[1] 谢委[1] 雷婷[1] 何维[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《中南药学》2004年第1期14-16,共3页Central South Pharmacy

基　　金：国家自然科学基金(3910877;39870872);卫生部科学研究基金(98-2-110)

摘　　要：目的 研究板蓝根抗内毒素活性成分丁香酸兔体内单剂量静脉注射动力学。方法 分别经兔耳静脉注射给药后,在不同时间经耳缘静脉取血,用高效液相色谱法测定血浆药物浓度.绘制药-时曲线,拟合房空模型,计算药物动力学参数。结果 静脉给药后,丁香酸在兔体内的药物动力学过程符合二室模型。其药动学参数为:α(0.11±0.14)min^(-1);β(0.04±0.01)min^(-1);V_0(373.8±119.2)mL;l_(1-2α)(9.7±7.8)min;l_(1-2β)(18.5±5.5)min;K_(21)(0.08±0.16)min^(-1);K_(10)(0.05±0.02)min^(-1);K_(12)(0.008±0.013)min^(-1);AUC_(0～155min)(6356.9±962.5)μg·min·mL^(-1);Cl(18.3±7.0)mL·min^(-1)。结论 本法简便、准确、灵敏度高、重现性好,可用于丁香酸体内动力学测定。OBJECTIVE To study the pharmacokinetics of syringic acid (SA) in rabbits by HPLC method. METHODS After the rabbits were injected sample solution via the ear vein, blood was also collected via the ear vein at different time. Then the concentration of SA in the blood was determined by HPLC and the concentration-time curve was mapped to simulate a compartment model. The pharmacokinetic parameters were calculated. RESULTS The concentration-time curve of SA inrabbits after iv fitted in a two-compartment open model. Pharmacokinetic parameters were: α(0.11±0.14) min-1;β (0.04±0.01) min-1 : Ve (373.8±119.2) mL; t1.2α (9.7±7.8) min; t1/2β (18.5±5.5) min; K21 (0.08±0.16) min-1; K10 (0.05±0.02) min-1 ; K12 (0.008±0.013) min-1; AUC0-155min (6356.9±962.5)μg·min·mL-1; Cl (18.3±7.0) mL·min-1 . CONCLUSIONS This method is simple, accurae, sensitive and reliable, and is suitable for pharmacokinetic study of syringic acid in vivo.

关 键 词：高效液相色谱法 板蓝根 丁香酸 药物动力学 

分 类 号：R927.2[医药卫生—药学]