甲硝唑对鸡离体外周血淋巴细胞的作用  

In Vitro Effects of Metronidazole on Peripheral Blood Lymphocyte(PBL)in Chicks

作　　者：乔彦良 付兴伦 盛英霞 张妍 杨汉春[3] 郭玉璞[3] 原培勋[4] 

机构地区：[1]山东神威动物保健有限公司,山东济南250014 [2]济南军区军事医学研究所,山东济南250014 [3]中国农业大学动物医学院,北京100094 [4]山东省畜牧兽医总站,山东济南250014

出　　处：《黑龙江畜牧兽医》2001年第11期1-3,共3页Heilongjiang Animal Science And veterinary Medicine

摘　　要：对咪唑类化合物甲硝唑对离体鸡外周血淋巴细胞 (PBL)增殖及伴刀豆球蛋白A(ConA)诱导细胞 (CIC)活性的作用并与咪唑类化合物左旋咪唑进行了比较 ;甲硝唑和左旋咪唑均以剂量依赖性方式促进鸡PBL的增殖 ;甲硝唑和左旋咪唑与ConA对促进鸡PBL增殖均呈现协同作用 ;甲硝唑和左旋咪唑对脂多糖 (LPS)激活鸡PBL均具有明显促增殖作用 ;甲硝唑和左旋咪唑均可逆转磷酸组胺和肾上肾对ConA和nLPS激活鸡PBL增殖的抑制作用 ;高浓度左旋咪唑 (10 0～ 40 0 μg/mL)可明显逆转氢化可的松对ConA激活鸡PBL增殖的抑制作用 ,各种浓度甲硝唑和左旋咪唑均可明显逆转氢化可的松对LPS激活鸡PBL增殖的抑制作用 ;甲硝唑和左旋咪唑均可促进CD+ 4细胞增殖 ,抑制CE+ 8细胞增殖 ,使CD+ 4/CD+ 8细胞比值升高 。In vitro effects of metronidazole on chick peripheral bloodlymphocyte(PBL) blastogenesis and ConA-induced cell(CIC) activitieswere reported and compared with levamisole.The results indicated that metronidazole increased PBL proliferation in a dose-dependent manner.Metronidazole increased the mitogenic response to ConA and LPS.Metronidazole reversed the suppresion response of histamine(HT) on theproliferation of PBL to ConA.Metronidazole antagonized the mitogenicinhibition of epinephrine on ConA-or LPS stimulated PBL.Metronidazoleeliminated the blastogenic suppression of hydrocortisone on LPS-agonized PBL,high concentration of metronidazole block its inhibitionon mitogenic response to ConA.Metronidazole increased the proliferationof CD +4 lymphocyte,decreased the blastogenesis of CD +8 lymphocyte,increased CD +4/C +8 ratio,and enhanced CIC positive activities on theproliferation response of lymphocyte to ConA.Levamisole,had verysimilar effects as metronidazole has.

关 键 词：甲硝唑 左旋咪唑 促增殖作用 PBL 逆转 外周血淋巴细胞 离体 活鸡 CIC 浓度 

分 类 号：S852[农业科学—基础兽医学] R781[农业科学—兽医学]