TT肝炎病毒及5种输血相关病毒感染云南省静脉吸毒者的分子流行病学调查研究  

PREVALENCE OF 5 KINDS OF VIRUS INFECTIONS AND THE SEQUENCE OF TT VIRUS AMONG IVDUS IN YUNNAN PROVINCE

作　　者：葛宪民 李丹亚[2] 曹坤 李勇强 黄果勇[4] 潘海东[4] 丁欣 沟上雅史 

机构地区：[1]广西壮族自治区工人医院,南宁530021 [2]广西医科大学第一附属医院,南宁530021 [3]日本名古屋市立大学医学院 [4]广西壮族自治区卫生防疫站,南宁530021

出　　处：《广西医科大学学报》2001年第2期155-160,共6页Journal of Guangxi Medical University

基　　金：卫生部攻关课题立项! (96-2 -3 4 6)

摘　　要：目的 :旨在探讨云南省静脉吸毒者 (intravenous drug users,IVDUs)的 HIV、HBV、HCV、HGV、HTL V和 TTV等6种病毒的感染现状及其 TTV核酸序列的特征。方法 :用选自云南省某戒毒所 15 8名 IVDUs的血清标本 ,用取自于 TTVDNA的基因组开放读码框架 1区 (ORF1 )合成的引物经半套式 PCR法检测 TTV DNA,并对 TTV DNA阳性标本全部进行了核酸序列分析和分子进化基因分析 ;还同步测试了 HIV、 HBV、 HCV、 HGV和 HTL V等 5种病毒感染的标志物 (分别用PA法、 PAH法、EIA法、WB法和 PCR法 )。结果 :TTV DNA阳性为 31/ 15 8(19.6 % ) ,抗 - HIV、 HBs Ag、抗 - HBs、抗- HBc、抗 - HCV、HGV- RNA和抗 - HTL V等 5种病毒的 7项标志物测试出阳性分别为 34 / 15 8(2 1.5 % )、 6 / 15 8(3.8% )、 98/15 8(6 2 .0 % )、 76 / 15 8(4 8.1% )、 136 / 15 8(86 .1% )、 6 5 / 15 8(4 1.1% )和 0 / 15 8(0 )。 31例 TTV DNA阳性者均合并上述5种病毒之一种以上的共同感染 ;31例 TTV DNA阳性者经测序和构建绘制分子进化同源序列树状图进行种系发生学分析表明 ,2 7/ 31(87.1% )为 TTV基因型 型中的 G1a、G1b、G1c、G1d型的四个基因亚型 ;4/ 31(12 .9% )为 TTV基因型2型中的 G2 a、 G2 b、 G2 c型的 3个基因亚型。结论Objective:To elucidate the prevalence of 6 kinds of virus (HIV,HBV,HCV,HGV,HTLV and TTV) infections and the sequence features of TTV among in travenous drug users (IVDUs) in Yunnan in southwest China Methods:Serum samples from 158 IVDUs in Yunnan Province were examined for TTV DNA by semi nested polymerase chain reaction (PCR) by using primers derived from the open reading frame (ORF 1) of TTV DNA The seroprevalence of viral markers of HIV,HBV,HCV,HGV and HTLV infection was also examined (by PA,PAH,EIA,WB,PCR) Sequence and a molecular evolutionary analysis was performed Results:Thirty one (20%) of the IVDUs were positive in TTV DNA,and 34(22%),6(4%),98(62%),76(48%),136(86%),65(41%) and 0(0%) were positive in anti HIV,HBsAg,anti HBs,anti HBc,anti HCV,HGV RNA and anti HTLV,respectively TTV infection was in all cases associated with co infection with 1 or more of the other above ementioned viruses Phylogenetic analysis revealed that the TTV isolates obtained in the study could be grouped mainly into 4 subgroups (Gla,Glb,Glc and Gld) of TTV genotype 1 among 27 IVDUs (87%),and could be grouped into 3 subgroups (G2a,G2b and G2c) of TTV genotype 2 among 4 IVDUs (13%) Conclusion:① New subgenotypes (G1c,G1d and G2c) of 3 kinds of TTV exist in China ②TTV infection is prevalent among IVDUs in Yunnan of China ③HIV,HBV,HCV and HGV infection is important among the IVDUs,but was not found HTLV infection among the IVDUs

关 键 词：云南省 静脉吸毒者 TT肝炎病毒 核酸序列分析 

分 类 号：R181.33[医药卫生—流行病学]