抗人红细胞表面抗原glycophorin A杂交瘤细胞株的建立  

Construction of Hybridoma Cell Strains Secreting McAbs to Human Erythrocyte Surface Antigen Glycophorin A

作　　者：蒋先敏[1] 于鲁东 程明[1] 

机构地区：[1]卫生部北京生物制品研究所,北京100024

出　　处：《中国生物制品学杂志》1993年第4期180-183,共4页Chinese Journal of Biologicals

摘　　要：应用杂交瘤技术,用纯化人红细胞表面抗原glycophorin A“N”型免疫纯系BALB/c小鼠,用glycophorin A“M”型作为固相抗原,用EIA间接筛选出识别“M”、“N”血凝抗原以外的抗glycophorin A的抗体,进而利用Coombs原理,加入抗免疫球蛋白,筛选出非凝集型抗glycophorin A抗体,得到61株非凝集型抗glycophorin A杂交瘤,其中12株有限稀释法克隆4次后得到5株稳定分泌非凝集型抗体的杂交瘤并进行初步鉴定。单抗上清血凝效价可达1:20～50,腹水血凝效价为1:800～1:1 600。The spleen cells of BALB/c mice immunized with human erythrocyte surface antigen glycophotin A type N were hybridized with SP 2/0 myeloma cells. Using glycophorin A type M as sol- id phase antigen, antibodies, except that against HA antigens type M and N, were screened out by in- direct EIA. By adding anti-immunoglobulin in the antibodies, 61 strains of nybridoma cell secreting non-agglutinating antibodies to glycophorin A were obtained. 12 of the 61 strains were cloned for 4 times by limited dilution, then 5 strains of bybridoma cell which could stably secrete non-agglutinating antibodies were screened ont and preliminarily detected. The results showed that the HA titres of McAbs in supernatant and ascite were 1: 20~1: 50 and 1: 800~1: 1600 respectively.

关 键 词：杂交瘤 红细胞抗原 血型糖蛋白 

分 类 号：R392－33[医药卫生—免疫学]