用蛋白A胶体金免疫电镜技术观察绵羊进行性肺炎病毒  

Observations on ovine progressive pneumonia virus by a protein A -colloidal gold immunoelectron microscopy

作　　者：胡尔马西[1] 林杰[1] 李建军[1] 胡泽渊[1] 罗琼[2] 

机构地区：[1]新疆畜牧科学院兽医研究所,乌鲁木齐830000 [2]新疆八一农学院

出　　处：《中国兽医科技》1993年第1期42-43,共2页Chinese Journal of Veterinary Science and Technology

摘　　要：本实验应用蛋白A胶体金免疫电镜技术与IEM、DEM、SPA-SPIEM比较观察了OPP-CMV_(40)-1株和CMV-33AN株。蛋白A胶体金方法所制备的样品在透射电镜下可观察到抗原—抗体复合物形成的抗体桥,在抗体桥上可看到病毒粒子。胶体金通过抗体桥分布在病毒颗粒的周围,胶体金周围还有多量病毒粒子。病毒颗粒呈球形,直径为80～100nm、外有一层囊膜,囊膜上有长约8nm的纤突。The CMV40-1 and CMV-33AN strains of ovine progressive pneumonia virus were observed fay a protein A-colloidal gold immunoelectron microscopy. Under a transmission electron microscope by amplifying the virus 3.0×105 to 5.0×105 times , the antibody bridge formed by the antigen-antibody complex and virions on it were seen, the virus particles were spherical, their diameters were 80 to 100 nm. There was a layer of envelope on which were about 8 nm long peplomers out the virions.

关 键 词：绵羊 肺炎病毒 蛋白A 胶体金免疫 

分 类 号：S858.265.3[农业科学—临床兽医学]