不明原因小鸡死亡和产蛋鸡输卵管囊肿病原诊断的研究  

Pathogenic diagnosis of chicks dying of unknown causes of disease and egg-laying hens with oviduct cyst I

作　　者：段跃进[1] 李英才[1] 杨学军[1] 高双娣[1] 帅永玉[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,730046

出　　处：《中国兽医科技》1993年第6期6-9,共4页Chinese Journal of Veterinary Science and Technology

摘　　要：自发病或死亡小鸡采取心、肝、脾、肺等脏器;对呈现腹部膨大或呈现企鹅姿势的病鸡采取腹水、输卵管囊肿液和心、肝、脾等脏器。将采集的病料直接涂片,以姬姆萨染色镜检,观察到多量衣原体原生小体,接种鸡胚卵黄囊培养出衣原体。用分得的衣原体代表株,人工感染试验证明,受感染小鸡可发生与自然病例相似的临床症状。分离株经鉴定为鹦鹉衣原体。Hearts, livers, spleens and lungs were collected from the affected or died chicks, and ascitic, fluid, fluid in oviduct cyst, hearts, livers and spleens were got from the affected egg-layi ng chickens with abdominal distention and penguin-like attitude, the samples of which were examined after Giemsa staining by microscopy, the elementary bodies of Chlamydia Sp were found. The microorganisms were grown by inoculation of the sample into normal chick embryo yolk sac, which were identified as Chlamydia psittaci. The artificial cases that were similar to natural ones were reproduced by inoculation of the isolates of C.psittaci into young chicks and 50 day-old hens.

关 键 词：鸡病 输卵管囊肿 鹦鹉衣原体 诊断 

分 类 号：S858.317.2[农业科学—临床兽医学]