理化诱变处理对水稻悬浮细胞系超微结构的影响  

Mutagenesis-induced Ultrastructural Changes in Suspension-cultured Rice Cells

作　　者：胡天赐[1] 高明尉[1] 成雄鹰[1] 滕俊琳[1] 高其康[1] 

机构地区：[1]浙江农业大学,杭州310029

出　　处：《中国水稻科学》1993年第2期71-76,共6页Chinese Journal of Rice Science

摘　　要：粳稻02428幼穗继代培养两年具有旺盛分裂能力的悬浮细胞系,有正常细胞结构,各种细胞器发育良好。但经500Rγ射线照射后,细胞结构产生较严重的损伤,表现线粒体数量减少,质体内淀粉粒积累,液泡增加;在照射后第6～12天,细胞结构逐渐模糊,细胞器消解;说明500 Rγ射线照射对细胞结构产生了不可逆转的损伤。5000 Rγ射线照射使悬浮细胞系产生致死损伤,更加速了细胞器解体和细胞死亡。1mmol/L和5mmol/L的叠氮化钠处理也使悬浮细胞系立即死亡。因此,具旺盛分裂能力的水稻悬浮细胞系对理化诱变处理的敏感性很高。The two-year old suspensions derived from the calli of japonica rice (CV. 02428) were normal in cell ultrastructures with well developed organs. However, the number of mitochondrion per cell decreased and starch grains in plastids and vacuoles in cytoplasm appeared 3 days after irradiation with 500R γ-rays. Cell structure changed dramatically with all cell organs disintegrated 6 to 12 days after the radiation indicating that the damage of cell ultrastructure caused by the irradiation is irreversible. Treatement with 5000R γ-rays brought about even greater damage to the suspensions, accelerating the disintegration of cell organs. The suspension cells treated with 1 mmol/L and 5 mmol/L of SA caused loss of growth ability. The suspension cells of 02428 showed a higher sensitivity to both physical and chemical mutagens as compared to the young panicle of the rice.

关 键 词：水稻 悬浮细胞系 超微结构 诱变 

分 类 号：S511.103.5[农业科学—作物学]